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In a comparison of 2 formalin killed vaccines, SHAWOGEN using an isolate (AVC U-13220) of Vibrio anguillarum (serotype 2) isolated from cod, and a commercial vaccine (VIBROGEN-2) prepared from 3 isolates (2 V. anguillarum, and V. ordalii) the vaccines were given i.p. with mild anaesthesia, by sprayi...
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A massive fish kill affecting exclusively common carp (Cyprinus carpio carpio) in the St. Lawrence River, Quebec, Canada, during the summer of 2001 was investigated by use of laboratory diagnostic methods and by an attempt to experimentally induce the disease. The ultimate causes of mortality were o...
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The factors affecting the horizontal transmission of infectious salmon anaemia virus (ISAV) among naive Atlantic salmon Salmo salar maintained in freshwater were examined. The ratio of injected to naive cohabitants was varied and mortality was monitored. Whether ISAV transmission in freshwater requi...
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Infection by a microsporidian of the genus Loma was found in gills of cod Gadus morhua. Xenomas contained parasites in multiple stages of development. Some spores looked empty and had everted polar tubes, which were either straight or coiled. These polar tubes were scattered throughout the xenoma cy...
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This case report provides pathologic and confirmatory molecular characterization of an outbreak of bumper car disease caused by the scuticociliate Anophryoides haemophila in American lobster Homarus americanus, from a commercial holding facility in Nova Scotia, Canada. Although sporadically and anec...
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Spores identified as Myxobolus neurobius were found in head homogenates of both wild young Atlantic salmon Salmo salar and Arctic char Salvelinus alpinus . Formalin-preserved spores from Atlantic salmon measured an average of 12.9 x 9.4 mu m and contained two polar capsules that measured 6.4 x 2.9 m...
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This case report provides pathological and confirmatory diagnostic documentation of the first reported clinical epizootic of infectious pancreatic necrosis (IPN) in farmed rainbow trout, Oncorhynchus mykiss, from central Mexico. Both the gross and microscopic pathology were consistent with IPN. A vi...
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Prolonged starvation resulting in sublethal condition factor values was hypothesized to have a detrimental effect on short-term growth capacity upon refeeding. Cod (Gadus morhua) were food-deprived and their length and mass measured before refeeding and after 3, 6, 9 and 12 weeks of ad libitum feedi...
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The phenotypic correlates of pathogenicity for Infectious salmon anemia virus (ISAV) in salmonid fishes have not been thoroughly studied to date. In this study, a comparison was made of 13 different strains of ISAV, isolated from different geographical regions between 1997 and 2004, for their infect...
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The riverine location of haematotoxic discharges was determined by exposing adult captive Atlantic salmon to River Don water in situ. Experiments were conducted using a control group and 4 test groups at different distances downstream from a paper mill and field drain discharges. Significant changes...
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Infectious salmon anaemia virus (ISAV) is a member of the family Orthomyxoviridae. Hybridization using riboprobes of segments 6 and 8 and histology were combined in situ to demonstrate the tissues that harbour ISAV during the clinical phase of ISAV infection in Atlantic salmon (Salmo salar L.) The c...
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This paper presents an overview of perkinsiosis in Salmo salar farmed in the Bay of Fundy, Canada, including information on the systematics of Perkinsus parasites, infection and disease caused by Perkinsus, lesions associated with the parasite in smolt and growout (market weight) salmon, differentia...
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A systemic infection of a Rickettsia-like organism (RLO) in cultured sea bass is described for the first time. In hatcheries, clinical signs were lethargy, inappetence and discoloration. Twenty days after transfer to sea cages from hatcheries where the disease existed, fish showed erratic and abnorm...
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A large-scale mortality of larval and juvenile halibut Hippoglossus hippoglossus occurred at a semi-commercial halibut farm in Atlantic Canada. Investigation of the cause revealed aquareovirus particles in necrotic liver tissue of affected fish. Cytopathic effect on CHSE-214 cell lines occurred from...
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The applicability of counter-immunoelectrophoresis (CIE) as an assay technique for the detection of precipitating antibody to R. salmoninarum (BKD) in the serum of chinook salmon ( Oncorhynchus tshawytscha ) was explored. Soluble antigen preparations of R. salmoninarum were obtained by precipitatio...
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BACKGROUND: Infectious salmon anaemia (ISA) is a viral disease of marine-farmed Atlantic salmon (Salmo salar) caused by ISA virus (ISAV), which belongs to the genus Isavirus, family Orthomyxoviridae. The virus is considered to be carried by marine wild fish and for over 25 years has caused major dis...
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Severe ulcerative, necrotizing bacterial dermatitis, panniculitis and myositis occurred in at least 6 Atlantic salmon cage sites in New Brunswick in 1990. Mortality was due to invasion by opportunistic bacteria, or osmotic failure due to excessive ulceration. Mortality up to 10% on individual cages ...
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Infectious salmon anaemia virus (ISAV) was isolated from farmed Atlantic salmon (Salmo salar) associated with an outbreak of haemorrhagic kidney syndrome in the Bay of Fundy, Canada. The virus induced cytopathic effects in salmon head kidney cell line SHK-1 from Atlantic salmon and was positively co...
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The cause of death in a postsmolt, Atlantic salmon population with elevated levels of mortalities was investigated. Diagnosis of a rickettsia-like organism was based on gross pathology, histopathology, differential staining, electron microscopy and fluorescent antibody tests. The course of the infec...
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Current understanding of the etiopathogenesis of infectious salmon anemia (ISA) virus (ISAV) infection in fish comes mostly from virus detection in homogenized tissues taken from ISA-suspected mortalities. This study combined in situ hybridization (ISH) and histology to demonstrate viral RNA transcr...