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Highly toxigenic as well as hypotoxigenic strains of Clostridium botulinum type C were isolated from 8 of 73 soil samples obtained from an avian botulism endemic area in Ontario. Amongst wild birds a high proportion of healthy pheasants carried the organism in their digestive tracts. One-third of th...
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The objectives of this study were to determine the carriage rate of Yersinia enterocolitica in the tonsils of slaughter hogs, and to characterize them with regard to phenotypic and virulence-associated properties. Of 202 pigs examined from an abattoir in Prince Edward Island, 85 were culture positiv...
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27 consecutive isolates of Salmonella recovered from animals between March 1990 and October 1991 were tested for susceptibility to 17 antibiotics using a standard disc diffusion assay. The majority of isolates originated from bovine (44.4%), avian (29.6%) and equine (11.1%) sources. The most common ...
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The plasmid profiles of oxytetracycline- and streptomycin-resistant isolates of Aeromonas salmonicida, Vibrio anguillarum, and Vibrio ordalii were examined by agarose gel electrophoresis. Bacterial isolates were from disease outbreaks in fish on the Atlantic and Pacific coasts. Resistant isolates we...
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Twenty strains of Clostridium botulinum type C, including 12 isolates from avian sources with varying toxigenic properties, were examined by electron microscope for the presence of bacteriophages. All toxigenic strains were infected with one or two types of phages. Three types of phages designated l...
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Culture filtrates of 28 Salmonella enteritidis isolates were tested for toxicity on Vero-, CHO-, and human foreskin fibroblast (HFF) cells. Cytopathic effects on HFF cells were extensive, and were observed even with some filtrates diluted 1:256. Vero cells showed effects with filtrates diluted up to...
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Fecal swabs obtained from a random sample of 1,000 beef slaughter steers and heifers from 123 Prince Edward Island (P.E.I.) farms were examined for the presence of Shiga toxin-producing Escherichia coli (STEC) using a Vero cell assay (VCA). Multiple isolates from each positive sample were tested sim...
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A fecal sample from a 4 1/2-year-old goat with a 2-month history of poor weight gain and diarrhea yielded a moderate growth of an organism resemblling Salmonella spp. on MacConkey agar. The organism was identified as Escherichia Fergusonii. The animal was euthanized. Samples of intesti...
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We report a rapid competitive enzyme-linked immunosorbent assay to screen Salmonella typhimurium strains for cholera-related enterotoxin antigens. Polymyxin B extracts of bacterial cells from syncase-glucose broth cultures of 7 of 15 strains gave positive results. The specificity of the test was con...
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Eighty-five blue mussels and 46 eastern oysters were collected from shellfish growing areas of 6 river systems in Prince Edward Island, and examined for aerobic and anaerobic bacterial flora. Animals were collected in different seasons during a 14-month period. Of a total of 907 isolates recovered, ...
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A microbiological assay was developed to quantify the concentrations of the quinolone antibiotics sarafloxacin and oxolinic acid in Atlantic salmon (Salmo salar) plasma. The assay was a modification of the Association of Official Analytical Chemists (AOAC) method for antibiotic concentrations in fee...
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Twenty-five strains of Salmonella typhimurium isolated in India were examined for the presence of cholera/coli-related enterotoxin antigen by a previously described latex particle agglutination test and by a newly developed immuno-dot-blot test using immunopurified goat antibody against the cholera-...
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The in vitro susceptibility of a total of 1819 bacterial isolates from canine and 103 isolates from feline otitis externa cases to 13 antimicrobial drugs over a 5-year period was evaluated. Among topically used drugs, 90% of isolates were susceptible to gentamicin. Susceptibility of Pseudomonas aeru...
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Fifty-nine Streptococcus suis type 2 isolates, recovered from pigs originating from Prince Edward Island, Canada, were tested for their susceptibility to eight antimicrobial agents by a disc diffusion method. All of the isolates were susceptible to penicillin and ampicillin. One third of isolates we...
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Fourteen human isolates of Campylobacter jejuni (13 pathogenic + 1 non-pathogenic) and two animal isolates were studied for the production of a toxin immunologically related to the heat-labile enterotoxin (LT) produced by a strain of Escherichia coli of human (H) origin using an immuno-dot-blot assa...