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Background: The proportion of geriatric horses within the equine population has increased in the past decade, but there is limited information on the immune function of these animals. Hypothesis: Aged horses will have a lesser increase in serum antibody response to vaccination. Animals: Thirty-four ...
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Serum antibody titers to bovine respiratory syncytial virus were determined for 559 cattle. Serum samples were obtained through the Minnesota State-Federal Brucellosis Laboratory and were collected over a 1-year period. Results of this study revealed an antibody prevalence of 65.5% to bovine respira...
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The general objective of this study was to evaluate a crude Ostertagia ostertagi antigen enzyme-linked immunosorbent assay (ELISA) for monitoring gastrointestinal parasites in lactating dairy cattle. A longitudinal study of gastrointestinal parasites in lactating dairy cows was carried out in 38 her...
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During a 19-month period, the agar gel immunodiffusion (AGID) test was used as an aid in differential diagnosis of paratuberculosis in 51 cattle with a history of chronic weight loss and/or chronic diarrhea. Thirty-three cattle were AGID test-positive for paratuberculosis. Twenty-eight cattle (87.5%...
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The induction of protection against xenoma development by vaccinated (previously exposed to a low-virulence strain of Loma salmonae; n=40) and naive (n=40) rainbow trout (Oncorhynchus mykiss) challenged with the virulent typical strain of L. salmonae, was determined. Prevalence of infection was lowe...
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An indirect haemagglutination test (IHA) and ELISA technique were developed to detect antibodies against Eimeria tenella. The ELISA technique was relatively easy to perform, more sensitive than the IHA test, and needed only a fraction of the antigen required for IHA. The highest titers using ELISA w...
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Quercetin was able to reduce the cytotoxic effect of T-2 mycotoxin on cultured murine thymocytes. When given to mice immediately before challenge with T-2 mycotoxins, quercetin significantly reduced mortality.
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An immunofluorescent antibody test (IFAT) developed for the diagnosis for plasmacytoid leukemia was evaluated against histology under field conditions. Previously published results from a laboratory evaluation indicated that the IFAT had a much higher sensitivity than did histology. One hundred seve...
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The meconium aspiration syndrome is an important cause of respiratory distress in newborn infants. Alveolar macrophages (AMs) provide a first line of defense in the lower respiratory tract against inhaled pathogens and particles such as meconium. In this study, we examined the effect of meconium on ...
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A lymphocyte stimulation test using antigens of Corynebacterium equi was used to compare the response of peripheral blood lymphocytes from foals with C equi pneumonia with those of clinically normal foals and adult horses. The test clearly distinguished infected foals from normal foals when tested i...
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Temperatures above 20 degrees C or below 9 degrees C interrupt the life cycle of the gill intracellular microsporidian parasite L. salmonae prior to sporogony, inhibiting the production of xenomas. This study intended to characterize this life-cycle failure. Juvenile rainbow trout were experimentall...
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Pooled seminal plasma obtained from six bulls was tested for its immunosuppressive activities in vitro on blastogenesis of bovine lymphocytes. Lymphocyte stimulation assay and mixed lymphocyte reaction were used as test procedures. Concanavalin A and phytohemagglutinin were utilized in the lymphocyt...
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Topics discussed in this review include classification of viruses, virus structure, epidemiology of viral infections, cell-mediated and humoral response, viral pathogenicity, viral defences and the detection, treatment and prevention of viral diseases..
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A genomic library of Sarcocystis cruzi sporozoite DNA was constructed in bacteriophage lambda gt10. Recombinant phages containing insert DNA were selected by growth on Escherichia coli strain C600 hflA150. Of 14 clones examined, 11 contained DNA inserts ranging in size from approximately 1.45 kiloba...
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An indirect Ostertagia ostertagi ELISA was used in late lactation milk samples from cows in confined and semi-confined dairy herds to determine if it could predict milk production response after endectocide treatment at calving. Holstein cows from 9 dairy farms from Prince Edward Island (PEI), 5 fro...
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Pasteurella haemolytica exerted a cytotoxic effect on bovine polymorphonuclear neutrophil leukocytes. This effect was less than that seen with cultured alveolar macrophages or peripheral blood monocytes. When alveolar macrophages were cultured with Pasteurella haemolytica, macrophages produced less ...
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The in vitro mitogen response of whole blood turkey lymphocytes to various concentrations of steroid hormones was evaluated. Corticosterone (COS) at concentrations between 1 and 80 ng/ml significantly suppressed the proliferative response (3H-thymidine incorporation) to phytohemagglutinin (PHA) and ...
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Six foals were inoculated intrabronchially with a suspension of Corynebacterium equi. Six weeks before this challenge, three foals were vaccinated with a C. equi bacterin. Three foals were unvaccinated controls. All foals developed a severe bronchopneumonia in the inoculated lung, indicating that va...
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An indirect enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against Ostertagia ostertagi using a crude adult worm antigen was evaluated using serum and milk samples from adult cows, as well as from bulk tank milk. Within and between plate repeatabilities were determined. In...
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The intragastric inoculation of a suspension of Corynebacterium equi on five consecutive days induced severe ulcerative colitis, typhlitis, and lymphadenitis of colonic and cecal nodes in two ponies necropsied three weeks after infection. No gross lesions were observed in two ponies necropsied ten d...
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Intradermal injection of phytohemagglutinin was used to evaluate the integrity of cell-mediated immunological reactions in Doberman puppies thought to be predisposed to demodicosis. Results indicate a statistically significant deficiency of cutaneous delayed response in these dogs when compared with...
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Cattle submitted to the University of Minnesota for surgical correction of left displaced abomasum (LDA) were examined for the in vitro phagocytic and bactericidal activities of their polymorphonuclear leukocytes (PMN). The PMN from cattle with LDA with or without concurrent infection had depressed ...