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In two trials, Arctic char (Salvelinus alpinus) semen was frozen in 0.5 mL straws using extenders consisting of 0.3 M glucose and 10%, 12.5% or 15% methanol. Cryopreserved semen was thawed by immersing straws in 25°C water for 17 s (11.6°C s-1) or in 5°C water for 60 s (3.3°C s-1). The viability...
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The effects of dietary lipid levels on growth, feed utilization, hepatosomatic index (HSI), liver lipid deposition and tissue fatty acid composition in haddock were investigated. Triplicate tanks of juvenile haddock (6.9 g) were fed graded levels of herring oil to supply 14, 16, 19 and 22% lipid (DM...
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Lupins were roasted in a flame roaster with an exit temperature of 105 degrees C. The effect of heating on protein solubility and rumen degradability of lupin were evaluated by chemical and Dacron bag procedures. Solubility of nitrogen in buffer was reduced from 69.8% in raw lupin to 35.8% in roaste...
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Lower fertility of preserved stallion semen may be caused by damage to the spermatozoa or premature capacitation during storage. We investigated the use of the flavonoid, quercetin, with a standard skim milk extender for storage of stallion spermatozoa to prevent premature capacitation and acrosome ...
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A trial in which roasted ground lupin, raw ground lupin or soyabean meal were fed to cows to establish possible effects on the fatty acid composition of milk (24 cows) and meat (28 cows) is reported. Results indicated that, in lupin-fed cows, even with a relatively low level of dietary fat, synthesi...
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The effects of dietary lipid from 4 experimental diets on the fatty acid composition and cholesterol content of spermatozoa and their plasma membranes and their consequences for sperm viability after cryopreservation were evaluated in rainbow trout. The 4 sources of lipid were herring oil (adequate ...
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Sweet white lupins (Lupinus albus) and a high protein variety of soyabeans, AC Proteus (HPSB), were roasted in an electric roaster at 300 degrees C for 1, 2, 3 or 4 min. Raw and roasted samples were analysed for total nitrogen, acid detergent insoluble N and protease insoluble N, and available rumen...
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This study investigated the relationship between fertility and fatty acid (FA) profiles of egg chorions, total egg, and sperm cells of Arctic char, Salvelinus alpinus (L.) broodstock housed in tanks and fed a commercial diet. Fertility of semen from individual males was assessed using pooled eggs, a...
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Seminal plasma protein of Arctic char Salvelinus alpinus was. characterized using sodium dodecyl sulfate (SDS) gel electrophoresis. Twelve protein bands with molecular weights of 7.2, 12.4, 15.3, 20.0, 20.4, 22.6, 39.4, 66.3, 74.0, 92.0, 94.5, and 130.1 kilodaltons (kDa) were detected. The effect of...
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The lipoprotein and lipid composition of the serum in postabsorptive haddock was characterized. A low level of very low density lipoproteins (VLDL; <50 mg/dl) was observed in serum. High density lipoprotein (HDL) was the predominant lipoprotein class followed by low density lipoproteins (LDL). Of...
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20 Holstein cows in midlactation were used in a Latin-square design to evaluate the nutritional quality of a high-protein soyabean (CP 45%) given raw or roasted. Treatments were: (1) control (soyabean meal); (2) conventional soyabean (Maple Isle), raw; (3) conventional soyabean, roasted; (4) high-pr...
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The effects of overfeeding by gastric intubation on body composition and energy metabolism of adult female rats were studied. There were significant linear increases in carcass DM, fat and energy during 10 days as daily metabolizable energy (ME) intake was increased from 160 to 300 kJ. Rats were giv...
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Dimethylacetamide (DMA) was compared with DMSO as a cryoprotectant for rainbow trout (Oncorhynchus mykiss) spermatozoa on the basis of motility, percentage of dead spermatozoa, enzyme leakage and fertility of thawed spermatozoa. DMA performed better (P<0.05) than DMSO for all traits..
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120 broiler-type [male][male] were kept under a regime of 23-h light:1-h darkness (23L:1D) at 54 lux from 1 day to 5 wk of age, when they were divided into 6 groups subjected to 12L:12D or 16L:8D at 5.4, 10.8 or 54 lux. Semen production and the fertility of semen diluted 1:3 in Lake's diluent were s...
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The total fatty acid composition of rainbow trout (Oncorhynchus mykiss) spermatozoa and seminal plasma was investigated in fresh and in stored semen using gas liquid chromatography. Triglycerides, phospholipids, and cholesterol were measured and the effect of fatty acids, triglycerides, and choleste...
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The objective of this study was to maintain the viability of chilled rainbow trout (Oncorhynchus mykiss) eyed eggs during storage using oxygenated perfluorochemical (PFC). Three trials were conducted using eggs at 161, 180 or 217 degree days (days from fertilization x incubation temperature in degre...
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The objective of this study was to evaluate the effects of feeding pigmented feed (50 ppm astaxanthin) to diploid or triploid Arctic charr, Salvelinus alpinus, on growth rate, color of fillets, and the variability of color within fillets. Arctic charr with an average weight of 419.9 g ± 83.3 g, inc...
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Effects of processing whole soyabean on growth characteristics, fatty acid composition and lipid peroxidation activity of beef tissue were investigated with 40 crossbred steers. The basal diet consisted of grass and corn silage, barley grain, vitamins and minerals. The dietary treatments were: raw s...
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80 ejaculates were obtained from each of 4 [male][male] from a line selected for post-thawing fertility duration of their inseminated spermatozoa, and the same number of ejaculates was obtained from 4 [male][male] of a randombred line. Semen was diluted with N,N-dimethylacetamide (DMA) or glycerol a...
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Droppings were collected every 2 days from hens on a standard maize and soya bean oilmeal diet with 17% protein. In DM crude protein was 27.5, Ca 8.5 and P 2.3%. The untreated excreta with 70% moisture was included as 30% in a diet at the expense of maize and oat husks to give a diet with 15% crude ...
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In Experiment 1, 24 multiparous cows in early lactation were given a basal diet for ad lib. intake consisting of 24.3% (DM) maize silage, 25.9% whole crop barley silage, 33.1% lucerne silage and 16.7% of a protein supplement. Raw barley grain, or barley flame roasted to reduce its rate of rumen degr...