Effect of methanol concentration and thaw rate on ...
|Title||Effect of methanol concentration and thaw rate on the viability and fertility of cryopreserved Arctic char, Salvelinus alpinus (L.), spermatozoa|
|Author(s)||G. Richardson, M. McNiven, N. Mansour|
|Abstract||In two trials, Arctic char (Salvelinus alpinus) semen was frozen in 0.5 mL straws using extenders consisting of 0.3 M glucose and 10%, 12.5% or 15% methanol. Cryopreserved semen was thawed by immersing straws in 25°C water for 17 s (11.6°C s-1) or in 5°C water for 60 s (3.3°C s-1). The viability of the frozen-thawed semen was measured by determining post-thaw motility and sperm membrane integrity. Two fertility trials were also conducted. There was no effect of trial or thaw rate on post-thaw sperm viability or fertility. Use of 15% methanol in the extender resulted in the highest overall percentage of sperm motility and fertility. Use of 12.5% methanol as a cryoprotectant resulted in a higher per cent post-thaw motility and a lower percentage of dead cells than did 10% methanol. Thus, levels of methanol higher than the commonly used 10% are beneficial for cryopreserving Arctic char sperm.|
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