Experimental infection of Peromyscus californicus ...
|Title||Experimental infection of Peromyscus californicus with Toxoplasma gondii|
|Author(s)||H. Dabritz, M. Miller, A. Packham, D. Rejmanek, C. Leutenegger, I. Gardner, E. Atwill, P. Conrad|
|Journal||Journal of Parasitology|
|Abstract||Eight female Peromyscus californicus were infected with 102 or 104 Toxoplasma gondii culture-derived tachyzoites (Type II or X) isolated from southern sea otters. All but 2 mice survived infection and developed antibodies to T. gondii. The 2 fatally infected mice were inoculated with 104 tachyzoites of the Type X strain. Parasite detection by immunohistochemistry (IHC) and DNA amplification with 2 polymerase chain reaction (PCR) methods was compared for brain, heart, lung, liver, spleen, biceps muscle, and tongue, at a mean of 41 days postinfection. Parasites were detected most commonly by IHC in spleen (8/8) and brain (6/8). DNA amplification by PCR was most successful from brain, heart, and spleen.|
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