An enzyme-linked immunosorbent assay (ELISA) for ...



Title An enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to channel catfish virus (CCV) in channel catfish
Author(s) S. A. Crawford, I. A. Gardner, R. P. Hedrick
Journal Journal of Aquatic Animal Health
Date 1999
Volume 11
Issue 2
Start page 148
End page 153
Abstract An ELISA was developed for the detection of antibodies against channel catfish virus (CCV) in the serum of channel catfish Ictalurus punctatus. The ELISA was used to test sera from 20 waterborne CCV-exposed juvenile channel catfish and 10 unexposed channel catfish for antibodies to CCV. Each serum was tested at a single dilution (1:10, volume:volume), and positive-negative cutoff values were established as the mean of negative sera plus either 2, 3, or 4 standard deviations. The resulting ELISA was highly reproducible and accurate with sensitivities and specificities of 100% and 90%, 95% and 90%, and 95% and 100%, respectively. Most fish seroconverted between 22 and 28 days after first exposure to CCV. This immunoassay is capable of examining large numbers of samples reliably and rapidly for detection and assessing prevalence of CCV exposure in channel catfish.
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