An enzyme-based formaldehyde assay and its utility ...
|Title||An enzyme-based formaldehyde assay and its utility in a sponge sterol biosynthetic pathway|
|Author(s)||R. G. Kerr, K. Kelly|
|Journal||Journal of Natural Products|
|Abstract||An enzyme-based assay has been developed and utilized to confirm the production of formaldehyde in the dealkylation of sterol side chains in a marine sponge. The enzyme used in the assay, formaldehyde dehydrogenase, is NAD+ dependent, and the assay measures the production of NADH by determining the increase in fluorescence at 460 nm.|
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