Detection of avian pneumovirus in tissues and swab ...



Title Detection of avian pneumovirus in tissues and swab specimens from infected turkeys
Author(s) J. C. Pedersen, D. A. Senne, B. Panigrahy, D. L. Reynolds
Journal Avian Diseases
Date 2001
Volume 45
Issue 3
Start page 581
End page 592
Abstract Conventional nested and TaqMan reverse transcription-polymerase chain reaction (RT-PCR) assays for the detection of avian pneumovirus (APV) were evaluated and compared with virus isolation (VI) for sensitivity and specificity. Respiratory tissues and tracheal swabs were collected from experimentally inoculated turkeys between 1 and 21 days postinoculation (DPI) and tested by all detection methods. APV was detected by both RT-PCR procedures as early as 1 and as late as 17 DPI whereas the virus was isolated only between 3 and 7 DPI. Pooled tracheal swab supernatants and dry swabs were excellent specimens for the detection of APV between 3 and 8 DPI. Turbinate and sinus specimens were the most productive samples over the entire collection period. Both RT-PCR assays were rapid and more sensitive than VI for the detection of APV in tissue and swab specimens from infected turkeys. RT-PCR allows for the rapid detection of APV from a variety of respiratory tissues as well as from dry swabs and tracheal swab supernatants. Antibodies to APV was detected in 50% of the sampled APV-inoculated birds at 8 and 9 DPI by enzyme-linked immunosorbent assay (ELISA). Early seroconversion (8-10 DPI) allows antibody detection to be used as a screening tool for APV. Rapid and sensitive detection methods are needed for APV, a highly contagious disease affecting USA poultry.

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