Evaluation of an automated colorimetric assay for ...



Title Evaluation of an automated colorimetric assay for the measurement of lipase activity in canine sera
Author(s) A. L. Mackenzie, Shelley A. Burton, D. W. Olexson, Barbara S. Horney, D. J. Honor, P. B. Maloney, S. D. Buczkowski
Journal Canadian Journal of Veterinary Research = Revue Canadienne de Recherche Veterinaire
Date 1996
Volume 60
Issue 3
Start page 205
End page 209
Abstract An automated colorimetric method for determining lipase activity in canine sera was evaluated for precision, linearity and correlation to existing assay methods. The colorimetric method was a commercial reagent that used a series of enzymatic reactions based on the hydrolysis of 1,2 diglyceride by pancreatic lipase. Within-run and between-run coefficients of variation were 7 y) dogs using this assay. It was concluded that the automated colorimetric assay was a reliable indicator of canine serum lipase activity and offered several advantages, including small sample volume and short analysis time.

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