Development of genomic probes to Sarcocystis cruzi ...



Title Development of genomic probes to Sarcocystis cruzi (Apicomplexa)
Author(s) Frederick S. B. Kibenge, Richard J. Cawthorn, D. Despres, P. K. McKenna, R. J. Frederick Markham
Journal Veterinary Parasitology
Date 1991
Volume 40
Issue 1-2
Start page 9
End page 20
Abstract A genomic library of Sarcocystis cruzi sporozoite DNA was constructed in bacteriophage lambda gt10. Recombinant phages containing insert DNA were selected by growth on Escherichia coli strain C600 hflA150. Of 14 clones examined, 11 contained DNA inserts ranging in size from approximately 1.45 kilobase (kb) to 6.18 kb. Insert DNA from four of these clones specifically hybridized to 32P-labelled S. cruzi merozoite DNA. One of these insert DNA, clone SL41, was selected and labelled with 32P. This probe did not hybridize with the other ten DNA inserts nor with bovine cellular DNA, but it hybridized with sporozoite, merozoite and bradyzoite DNA preparations. The SL41 probe could detect merozoite DNA in as little as 17 ng total DNA. Genomic probes detecting developmental stages of Sarcocystis spp. could provide an improved means is diagnosis of acute bovine sarcocystosis.

Using APA 6th Edition citation style.

[Page generation failure. The bibliography processor requires a browser with Javascript enabled.]

Times viewed: 531

Adding this citation to "My List" will allow you to export this citation in other styles.