Efficacy of vaccination in preventing giardiasis in ...



Title Efficacy of vaccination in preventing giardiasis in calves
Author(s) F. D. Uehlinger, R. M. O'handley, Spencer J. Greenwood, N. J. Guselle, L. J. Gabor, C. M. Van Velsen, R. F. Steuart, H. W. Barkema
Journal Veterinary Parasitology
Date 2007
Volume 146
Issue 1-2
Start page 182
End page 188
Abstract The objective of this study was to evaluate the efficacy of a vaccine in the prevention of Giardia duodenalis infection in calves. Six 2-week old calves were vaccinated subcutaneously with a sonicated G. duodenalis trophozoite vaccine. Six 2-week old control calves received a subcutaneous injection of sterile phosphate-buffered-saline mixed with adjuvant. Injections were repeated after 28 days. Eleven days after the second injection, calves were challenged orally with 1x10(5) purified G. duodenalis cysts from a naturally infected calf. Throughout the study, fecal samples were collected at regular intervals and examined for the presence of G. duodenalis cysts. Blood samples were collected weekly until G. duodenalis challenge and bi-weekly following challenge. Calves were euthanized 14 days after challenge and G. duodenalis trophozoites within the small intestines were enumerated. Serum antibody titers were significantly higher in vaccinated compared to non-vaccinated calves. Vaccinated calves tended to excrete more G. duodenalis cysts in their feces than non-vaccinated calves. The number of trophozoites in the small intestine was not different between vaccinated and non-vaccinated calves. Changes consistent of moderate enteritis were found in the intestines of one vaccinated and one non-vaccinated calf. Despite a serological immune response following vaccination, this vaccine was not efficacious in preventing giardiasis or reducing cyst shedding in calves.
DOI 10.1016/j.vetpar.2007.01.013
Use/Reproduction Contact Author

Using APA 6th Edition citation style.

[Page generation failure. The bibliography processor requires a browser with Javascript enabled.]

Times viewed: 412

Adding this citation to "My List" will allow you to export this citation in other styles.