The plasmid profiles of fish pathogenic isolates of ...

Description

Citation

Title The plasmid profiles of fish pathogenic isolates of Aeromonas salmonicida, Vibrio anguillarum, and Vibrio ordalii from the Atlantic and Pacific coasts of Canada
Author(s) J. S. Giles, H. Hariharan, S. B. Heaney
Journal Canadian Journal of Microbiology
Date 1995
Volume 41
Issue 3
Start page 209
End page 216
Abstract The plasmid profiles of oxytetracycline- and streptomycin-resistant isolates of Aeromonas salmonicida, Vibrio anguillarum, and Vibrio ordalii were examined by agarose gel electrophoresis. Bacterial isolates were from disease outbreaks in fish on the Atlantic and Pacific coasts. Resistant isolates were examined when grown in the presence and absence of antibiotic. Alkaline lysis methods were used for plasmid isolation. Vibrio spp. were predominantly plasmidless, except for a 47-kilobase (kb) plasmid. Atlantic coast isolates of A. salmonicida possessed four or six plasmids, with four smaller plasmids ranging in size from 4.3 to 8.1 kb being consistently observed. The plasmid profiles of antibiotic-sensitive ATCC strains were identical. The plasmid profiles of the Pacific coast isolates of A. salmonicida varied slightly from those of the Atlantic coast isolates with six plasmids observed, ranging in size from 4.2 to 8.9 kb. Resistance to the antibiotics was not altered following plasmid curing experiments and resistance was not transferable to Escherichia coli. Thus, resistance to oxytetracycline and streptomycin did not appear to be plasmid mediated.

Using APA 6th Edition citation style.

[Page generation failure. The bibliography processor requires a browser with Javascript enabled.]

Times viewed: 378

Adding this citation to "My List" will allow you to export this citation in other styles.