Agarose gel electrophoresis of alkaline phosphatase ...

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Title Agarose gel electrophoresis of alkaline phosphatase isoenzymes in the serum of hyperthyroid cats
Author(s) Barbara S. Horney, A. J. Farmer, D. J. Honor, A. MacKenzie, Shelley A. Burton
Date 1994
Volume 23
Issue 3
Start page 98
End page 102
Abstract Cats with hyperthyroidism [(increased serum thyroxine (T(4))] commonly have increased serum alkaline phosphatase (ALP) activity in addition to other serum biochemical abnormalities. Serum biochemical profiles were obtained from 10 hyperthyroid cats which had increased serum ALP. Agarose gel electrophoresis of serum from these cats was performed and stained for alkaline phosphatase activity. Alkaline phosphatase activity was calculated for each of the separate bands obtained, and the results were compared to those of tissue extracts, serum from normal cats, and serum from normothyroid cats with increased serum ALP activity. The hyperthyroid cats had increased ALP activity in bands corresponding to isoenzymes originating in the liver, bone, and an unidentified tissue source.
PubMed ID 12666024

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