Characterization of monoclonal antibodies against ...



Title Characterization of monoclonal antibodies against bovine herpesvirus 1 gD fusion protein expressed in E. coli
Author(s) J. R. Lyaku, P. K. McKenna, R. A. Fredrickson, Frederick S. B. Kibenge
Journal Journal of Virological Methods
Date 1999
Volume 83
Issue 1-2
Start page 83
End page 89
Abstract A total of 20 hybridoma cell lines secreting monoclonal antibodies (MAbs) against E. coli expressed bovine herpesvirus-1 (BHV-1) gD fusion protein were produced following the fusion of Sp2/0 myeloma cells with splenocytes from BALB/c mice immunized previously with immunoaffinity purified BHV-1 gD fusion protein. An indirect fluorescent antibody test (IFAT) using BHV-1 infected MDBK cells was used for the selection of positive hybridomas secreting specific antibody. The monoclonal antibody isotypes were 11 IgM, six IgG2b, one IgG1 and two IgG3. All MAbs reacted positively with the E. coli expressed BHV-1 gD fusion protein, BHV-1 infected MDBK cell lysates and PCR BHV-1 gD transcription-translation polypeptide antigens by an ELISA.

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