Altering the cellular location of an antigen ...
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Citation
| Title | Altering the cellular location of an antigen expressed by a DNA-based vaccine modulates the immune response |
| Author(s) | P. Lewis, H. van Drunen Littel-van den, L. Babiuk |
| Journal | Journal of virology |
| Date | 1999 |
| Volume | 73 |
| Issue | 12 |
| Start page | 10214 |
| End page | 10223 |
| Abstract | The potential for DNA vaccines encoding mutated versions of the same antigen to modulate immune responses in C3H/HeN mice was investigated. We created expression plasmids that encoded several versions of glycoprotein D (gD) from bovine herpesvirus 1, including authentic membrane-anchored glycoprotein (pSLRSV.AgD), a secreted glycoprotein (pSLRSV.SgD), and an intracellular protein (pSLRSV.CgD). Immunization of an inbred strain of mice with these plasmids resulted in highly efficacious and long-lasting humoral and cell-mediated immunity. We also demonstrated that the cell compartment in which plasmid-encoded gD was expressed caused a deviation in the serum immunoglobulin (Ig) isotype profile as well as the predominant cytokines secreted from the draining lymph node. Immunization of C3H/HeN mice with DNA vaccines encoding cell-associated forms of gD resulted in a predominance of serum IgG2a and gamma interferon-secreting cells within the spleens and draining lymph nodes. In contrast, mice immunized with a secreted form of this same antigen displayed immune responses characterized by greater levels of interleukin 4 in the draining lymph node and IgG1 as the predominant serum isotype. We also showed evidence of compartmentalization of distinct immune responses within different lymphoid organs. |
| ISSN | 0022-538X |
Using APA 6th Edition citation style.
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