Agar gel immunodiffusion test for diagnosis of clinical paratuberculosis in cattle

Agar gel immunodiffusion test for diagnosis of ...

Title	Agar gel immunodiffusion test for diagnosis of clinical paratuberculosis in cattle
Author(s)	D. Sherman, R. Markham, F. Bates
Journal	Journal of the American Veterinary Medical Association
Date	1984
Volume	185
Issue	2
Start page	179
End page	182
Abstract	During a 19-month period, the agar gel immunodiffusion (AGID) test was used as an aid in differential diagnosis of paratuberculosis in 51 cattle with a history of chronic weight loss and/or chronic diarrhea. Thirty-three cattle were AGID test-positive for paratuberculosis. Twenty-eight cattle (87.5%) yielded Mycobacterium paratuberculosis from bacterial culture of feces. Four cattle were confirmed as having paratuberculosis on necropsy. One had a negative fecal culture but was lost to follow-up. Thus, 32 of the 33 AGID test-positive cattle (96.9%) were confirmed as paratuberculous by culture or necropsy. Of the 18 cattle that were AGID test-negative, 17 (94%) also were culture-negative after 12 to 20 weeks' incubation of the culture tubes. Direct fecal smears from 29 confirmed paratuberculous cattle were examined. Twenty-three (79.3%) had smears positive for M paratuberculosis. Fifteen culture-negative cattle were examined by direct smear; the results were negative for all.
ISSN	0003-1488

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