Comparison of ELISA and immunofluorescence assays ...

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Title Comparison of ELISA and immunofluorescence assays for detection of feline leukemia virus antigens in blood of cats
Author(s) V. M. Hirsch, G. P. Searcy, James E. C. Bellamy
Journal Journal of the American Animal Hospital Association
Date 1982
Volume 18
Issue 6
Start page 933
End page 938
Abstract Two methods for detecting feline leukaemia virus (FeLV) antigen in peripheral blood were compared in 128 cats: immunofluorescence assay (IF) and an enzyme-linked immunosorbent assay (ELISA). Both healthy cats and cats with a variety of diseases, including lymphosarcoma and myeloproliferative disorders were tested. The results were correlated with haematological findings and FOCMA titres. It was found that the ELISA method detected all IF positive cats, but a significant number (13/30) of cats were positive by the ELISA method alone. The majority of cats that were IF negative and ELISA positive had haematological or pathological evidence of FeLV-related disease..

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