Use of a complex virological technique for direct ...

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Title Use of a complex virological technique for direct demonstration of PI-3 and IBR viruses in the organs of infected calves
Author(s) J. Mensik, Z. Pospisil, A. Suchankova, Arnost Cepica, V. Rozkosny, B. Smid, M. Machatkova
Journal Veterinarni Medicina
Date 1975
Volume 20
Issue 10/11
Start page 609
End page 622
Abstract The fluorescent antibody technique or horse-radish peroxidase labelled antibodies demonstrated parainfluenza-3 virus in nasal touch preparation and in sections of the nasal and tracheal mucosa and lungs within the first seven days after experimental intratracheal infection. Bovine foetal organ cultures gave better isolation of PI-3 virus than established bovine foetal cell lines. Bovine rhinotracheitis virus was isolated in foetal cell and organ cultures at 3, 7 and 11 days after experimental infection. PI-3 and IBR virus in mixed infections were demonstrated by electron microscopy using negative staining..

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