The detection of precipitating antibody to ...



Paper title The detection of precipitating antibody to Renibacterium salmoninarum in chinook salmon by counter-immunoelectrophoresis: Proceedings of a Symposium organized by the International Association of Biological Standardization and held at the National Fish Health Research Laboratory, Leetown, W.Va. USA. April 26-30, 1981
Paper author(s) David B. Groman
Proceedings title Developments in biological standardization. Fish biologics: Serodiagnostics and Vaccines
Date 1981
Abstract The applicability of counter-immunoelectrophoresis (CIE) as an assay technique for the detection of precipitating antibody to R. salmoninarum (BKD) in the serum of chinook salmon ( Oncorhynchus tshawytscha ) was explored. Soluble antigen preparations of R. salmoninarum were obtained by precipitation with saturated ammonium sulphate and 10% trichloroacetic acid. These antigen precipitates were screened by CIE against goat anti-BKD serum and sera from chinook salmon experimentally innoculated with heat killed R. salmoninarum and Freund's complete adjuvant. Preliminary results indicated that CIE was successful in detecting both of the antigen preparations as well as blood borne R. salmoninarum when screened against goat anti-BKD. No precipitin lines were noted, however, when chinook serum was substituted for the goat antiserum. These inconclusive results suggest that further research is warranted before CIE can be used for the detection of precipitating antibody to R. salmoninarum in chinook salmon.
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