Comparative analysis of a monoclonal-antibody-based ...



Title Comparative analysis of a monoclonal-antibody-based immunofluorescence test for plasmacytoid leukemia in chinook salmon Oncorhynchus tshawytscha
Author(s) G. C. Newbound, R. J. Frederick Markham, M. L. Kent, David J. Speare, M. A. Thorburn
Journal Diseases of Aquatic Organisms
Date 1995
Volume 21
Issue 2
Start page 123
End page 130
Abstract Plasmacytoid leukaemia (PL) is a lymphoproliferative disorder that affects netpen-reared chinook salmon in British Columbia, Canada. The only diagnostic test for PL is histological evaluation of suspect fish. Monoclonal antibodies (MAbs) previously reported to react with tissues of PL-affected fish were used in a quantitative indirect fluorescence antibody (IFA) test. These MAbs reacted with a 75 kDa antigen that is highly expressed in tissues of fish with PL. Using experimentally infected fish, the sensitivity, specificity, and predictive values of the IFA test were compared with those of the standard histological test. The IFA test was found to have a sensitivity of 72%, which was significantly higher than that of histological examination, and could detect experimental infection at 3 wk after injection, earlier than histological examination. The sensitivity and practicality of the IFA test will enable it to be a useful screening test for the detection of PL..
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