Effects of a pathogenic canine herpesvirus on ...



Title Effects of a pathogenic canine herpesvirus on established thyroid adenoearanoma cell cultures
Author(s) Gerald R. Johnson, A. Koestner, O. Kindig, J. A. Shad duck
Journal Pathologia Vet
Date 1969
Volume 6
Start page 289
End page 308
Abstract Early lesions in these cultures (3-10 hours after virus inoculation) consisted of nucleolar sweating and disruption; there was loss of RNA from the nucleo-lus and segregation of nueleolar components (demonstrated by electron microscopy). During the middle phase (10-34 hours) three types of eosinophilic intranuclear inclusion appeared; the inclusions seemed to be formed by progressive transformations. Viral antigen was demonstrated in the nucleus by immuno-fluorescent staining, with viral replication observed at the same time by electron microscope. The late phase (34-72 hours) was manifested by cellular degeneration and viral release into the cytoplasm..

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