Electrophoretic and immunochemical analyses of the ...
|Title||Electrophoretic and immunochemical analyses of the lipopolysaccharides from various strains of Aeromonas hydrophila|
|Author(s)||J. Dooley, R. Lallier, D. Shaw, T. Trust|
|Journal||Journal of Bacteriology|
|Abstract||Lipopolysaccharides isolated from strains of Aeromonas hydrophila, virulent for fish and which autoaggregate during growth in static broth culture, were examined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The lipopolysaccharides contained O-polysaccharide chains of homogeneous chain length. Two of the strains produced a surface protein array, and immunofluorescence and phage-binding showed that a number of these O-polysaccharide chains of homogeneous length traversed the protein array and were exposed on the cell surface. There were three epitopes on the polysaccharide portion of this homogenous-chain-length lipopolysaccharide morphotype. One epitope was species serogroup specific and reactive by immunoblotting. This epitope was not present on the heterogeneous-chain-length O polysaccharides of non-autoaggregating strains of A. hydrophia examined. The second epitope was conformation dependent and cross-reactive with an epitope on the homogenous-chain-length O polysaccharides of Aeromonas salmonicida lipopolysaccharide. The third epitope was recognized by a monoclonal antibody and appeared to involve that region of the A. hydrophila and A. salmonicida lipopolysaccharide molecules which contained the O-polysaccharide-core oligosaccharide glycosidic linkage..|
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