Feulgen iron propiono carmine and cupra ammonium in ...



Title Feulgen iron propiono carmine and cupra ammonium in preparing algal chromosomes for light microscopy
Author(s) Louis A. Hanic
Journal Stain Technology
Date 1979
Volume 54
Issue 3
Start page 129
End page 134
Abstract A method for obtaining algal chromosomal preparations is described employing the Fuelgen method for DNA staining, Fe-propionocarmine as an enhancing stain, and cupra-ammonium to remove cell wall material. Fe-propionocarmine applied as a gradient to the slide provides cells stained with the Feulgen stain alone or with the Fuelgen Fe-propionocarmine stain, thereby facilitating useful comparison. Where dilute, the Fe-propionocarmine enhances nuclear staining without staining other organelles; where more concentrated, it also stains the nucleolus, spindle, spindle polar bodies, pyrenoid and protoplast. Treatment with cupra-ammonium, to remove polysaccharide wall material, followed by neutralization with propionocarmine, enables thinner squashes and better chromosome spreads without loss of differential staining. Preparations mounted in euparal are long-lasting.

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