Tetrazolium-based cytotoxicity assay to determine ...
|Title||Tetrazolium-based cytotoxicity assay to determine anti-protozoal activity against the scuticociliate Anophryoides haemophila|
|Author(s)||Alastair E. Cribb, B. Despres, Richard J. Cawthorn|
|Journal||Diseases of Aquatic Organisms|
|Abstract||A tetrazolium-based cytotoxicity assay was modified to determine the anti-protozoal activity of compounds towards the lobster parasite A. haemophila. To obtain reproducible results, A. haemophila maintained in continuous culture were preferable to freshly isolated A. haemophila. It was also found that the ciliates must be replicating to produce reproducible results. Maximum anti-protozoal activity was evident after a 24 h incubation period. Oxytetracycline was relatively inactive, while high concentrations (1 mM) of formaldehyde and sulphaquinoxaline were required to produce 50% cytotoxicity. In contrast, 100 micro M lasalocid produced 92+or-4% and 100 micro M pyrimethamine produced 84+or-6.5% cytotoxicity after 24 h. It is concluded that this objective assay provides a rapid means of screening compounds to identify those with potential in vivo activity against Anophryoides haemophila..|
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