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The multilobular tumor of bone (MTB) is considered as a slow-growth malignant tumor, which generally occurs on the cranium and facial bones of dogs, although it has been reported in other domestic animals. This study presents two cases of MTB in dogs. The first case was manifested in an 8-year-old m...
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Current understanding of the etiopathogenesis of infectious salmon anemia (ISA) virus (ISAV) infection in fish comes mostly from virus detection in homogenized tissues taken from ISA-suspected mortalities. This study combined in situ hybridization (ISH) and histology to demonstrate viral RNA transcr...
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Routine laboratory diagnosis of infectious salmon anaemia virus (ISAV) infection is primarily by reverse transcription polymerase chain reaction (RT-PCR) because of the high sensitivity and rapid turnaround time of the test. This paper describes methods for highly reproducible absolute viral load me...
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A sebaceous gland carcinoma was diagnosed in an adult African hedgehog (Atelerix albiventris). The neoplasm,was localized in the subcutis of the cervicall region and extendend towards the axilar region and along the thorax. The animal was admitted for surgical removal of the tumor but died during th...
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From winter 1997 to summer 1998, an epizootic of salmonellosis affected several species of songbirds over a large area of the eastern North American continent. This article describes the details of this epizootic in the Canadian Atlantic provinces, based on laboratory examination of dead affected bi...
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A cross-sectional study was undertaken to determine the prevalence and distribution of Salmonella serotypes from apparently healthy slaughtered sheep and goats from November 2002 to May 2003 at Debre Zeit abattoir, Ethiopia. From a total of 107 slaughtered animals (60 goats and 47 sheep), 642 sample...
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An automated colorimetric method for determining lipase activity in canine sera was evaluated for precision, linearity and correlation to existing assay methods. The colorimetric method was a commercial reagent that used a series of enzymatic reactions based on the hydrolysis of 1,2 diglyceride by p...
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Three calves from a herd of beef cattle were examined because of disproportionate dwarfism and excessive extension of metacarpophalangeal and metatarsophalangeal joints. Abnormalities had been noticed at birth, 1 to 6 days earlier. A thorough herd investigation revealed that 16 calves born to 70 mul...
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Severe generalized degenerative myopathy was diagnosed in a population of captive bred satanic leaf-tailed geckoes (Uroplatus phantasticus). The diagnosis was based on characteristic histological changes and response to dietary therapy. This is the first reported case of nutritional myopathy in the ...
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Peripheral blood lymphocytes, intraepithelial lymphocytes from the small intestine and lymphocytes from the thymus, spleen, mesenteric lymph nodes and Peyer's patches from 5 young adult pigs were used as effector cells in a spontaneous cell-mediated cytotoxicity chromium release assay against PK-15 ...
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OBJECTIVE: To determine the FIV status of Australian cats with lymphosarcoma and relate this to patient characteristics, tumour characteristics (tissue involvement, histological grade and immunophenotype), haematological and serum biochemical values and FeLV status of affected cats. DESIGN: Prospect...
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Background: The proportion of geriatric horses within the equine population has increased in the past decade, but there is limited information on the immune function of these animals. Hypothesis: Aged horses will have a lesser increase in serum antibody response to vaccination. Animals: Thirty-four ...
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The potential for cross-transmission of Perkinsus karlssoni from bay scallops Argopecten irradians to various native Atlantic Canadian species of shellfish was investigated. Lateral proximal transmission was investigated whereby infected moribund bay scallops were maintained with uninfected individu...
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Effective methods of traceability are urgently required for use in research as well as in different types of aquaculture operations and to control trade in aquatic animals and products. In regard to the marking of fish, many different tagging methods have been described and the method to be used dep...
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In the summer of 1999, the incidence of Salmonella enterica serotype infantis infections in Alberta rose dramatically. Subsequent laboratory and epidemiological investigations established that an outbreak of human disease caused by this organism was occurring across Canada and was associated with pe...
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A reverse transcription-polymerase chain reaction (RT-PCR) assay for the detection of avian pneumovirus (APV), Colorado strain (US/CO), was evaluated for sensitivity and specificity. The single-tube RT-PCR assay utilized primers developed from the matrix (M) gene sequence of the US/CO APV. The RT-PC...
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Objectives: Methods: Results: Conclusions: The aim of this study was to investigate the feasibility and efficacy of antisense-mediated gene silencing by peptide nucleic acid (PNA) for specific inactivation of the CmeABC multidrug efflux transporter in Campylobacter jejuni.PNA was designed to bind to...
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Serum antibody titers to bovine respiratory syncytial virus were determined for 559 cattle. Serum samples were obtained through the Minnesota State-Federal Brucellosis Laboratory and were collected over a 1-year period. Results of this study revealed an antibody prevalence of 65.5% to bovine respira...
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The general objective of this study was to evaluate a crude Ostertagia ostertagi antigen enzyme-linked immunosorbent assay (ELISA) for monitoring gastrointestinal parasites in lactating dairy cattle. A longitudinal study of gastrointestinal parasites in lactating dairy cows was carried out in 38 her...