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Background: The proportion of geriatric horses within the equine population has increased in the past decade, but there is limited information on the immune function of these animals. Hypothesis: Aged horses will have a lesser increase in serum antibody response to vaccination. Animals: Thirty-four ...
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The presence of dendritic cells in fish is studied with immunohistochemistry using a commercially available antibody developed against Langerin/CD207 present in human Langerhans cells. Langerin/CD207, a protein known to be associated with the development of Birbeck granules in human and murine syste...
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A dog adopted as a stray in Spain and then brought to Canada 4 years prior to presentation was evaluated for polyarthritis. An electrophoresis showed a marked polyclonal gammopathy and synovial smears contained leishmanial organisms within macrophages.
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The prevalence of Giardia duodenalis, Cryptosporidium spp. and other intestinal parasites was determined in dogs <1 year old from Prince Edward Island, Canada. Fecal samples were collected from the local animal shelter (n=62), private veterinary clinics (n=78) and a pet store (n=69). Intestinal para...
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Studies on the microanatomy of the mucus-rich biofilm surface of normal or damaged teleost skin tissue have been limited because conventional fixation regimes do not effectively retain mucus during tissue preparation. A non-aqueous fixation method, based on a technique devised to retain airway mucou...
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Infectious salmon anaemia (ISA) virus (ISAV) is a fish orthomyxovirus that has recently been assigned to the new genus Isavirus within the family Orthomyxoviridae. It possesses the major functional characteristics of the virus family including haemagglutinating, receptor destroying enzyme (RDE), and...
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A thymoma was tentatively diagnosed by radiographic and cytologic examination in a dog with hypercalcemia and elevated serum parathyroid hormone-related protein (PTHrP) concentration. Following surgical excision, the diagnosis of thymoma was confirmed via histopathologic examination, the hypercalcem...
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Domoic acid (DOM), the toxin involved in amnesic shellfish poisoning, was presented to live mussels (at 5 degree C, 28 ppt) in dissolved form (125 nM) and as food encapsulated in liposomes. Less than 1% of dissolved DOM and up to 6% of food-borne DOM was incorporated into mussel tissues. DOM absorbe...
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A Clydesdale mare was examined for weight loss, inappetence, ptyalism, and dysphagia. The main abnormality revealed by serum biochemistry was a marked hyperglobulinemia, and protein electrophoresis revealed a monoclonal gammopathy in the gamma region. The urine was positive for Bence Jones proteins....
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An optimal in situ hybridization protocol is described for the detection of gene expression of a structural protein unique to lampreys, lamprin, in the cartilages of prolarval, metamorphic and adult sea lamprey, Petromyzon marinus. A 156 bp antisense RNA probe labeled with (35)S-UTP was transcribed ...
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The adult American lobster (Homarus americanus) is susceptible to few naturally occurring pathogens, and no viral pathogen is known to exist. Despite this, relatively little is known about the H. americanus immune system and nothing is known about its potential viral immune response. Hundreds of rur...
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In two variants of Loma salmonae that have specificity for rainbow trout Oncorhynchus mykiss (OA variant) and specificity to brook trout Salvelinus fontinalis (SV variant), the parasitophorous vacuole forms at the onset of sporogony. In the OA variant the merogonial stage is bound by a single plasma...
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Chloramine-T is a widely used prophylactic and therapeutic agent in freshwater aquaculture (mainly against bacterial gill disease). The effects of repeated intermittent exposure of healthy rainbow trout (Oncorhynchus mykiss) fingerlings to sublethal concentrations of chloramine-T (0,5, 10, or 20 mg/...
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The ontogeny of major histocompatibility class II antigens in small intestine enterocytes of postnatal C3H/He mice was investigated. Cryosections of duodenal, jejunal, and ileal segments from 7-, 14-, 16-, 20-, 21-, 23-, 25-, 27-, 28-day-old and 7-week-old mice were stained for the class II antigens...
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The qualitative and quantitative expression of major histocompatibility class II antigens was investigated in the absorptive epithelium of the duodenum, jejunum, and ileum from mice of C3H/He (H-2k haplotype) and C57BL/6 (H-2b haplotype) strains by peroxidase-antiperoxidase labelling and image analy...
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Crenosoma vulpis and Eucoleus aerophilus are nematode parasites that can cause verminous pneumonia in wild carnivores. There is a paucity of information regarding the distribution of parasites in the lungs and the relationship between histopathological and parasitological diagnoses in naturally infe...
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The effects of dexamethasone (dex) treatment on infections with the microsporidian parasite, Loma salmonae and the effects of dex on initiation of the adaptive immune response were investigated in rainbow trout, Oncorhynchus mykiss experimentally infected with the parasite. Dex treatment resulted in...
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The early ultrastructural stages of Loma salmonae were studied in the gills of experimentally infected rainbow trout. No parasitic stages were identified during the first 2 wk of the infection. By week 3 postexposure (PE), uninucleate and binucleate meronts were recognized within host cells (no xeno...
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Life-span and reproductive capacity of sea lice, Lepeophtheirus salmonis, were investigated in artificial seawater (Instant OceanReg.) under laboratory conditions. Sea lice were maintained on Atlantic salmon (Salmo salar) in recirculation systems for as long as 7 months. During this period sea lice ...