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Fecal swabs obtained from a random sample of 1,000 beef slaughter steers and heifers from 123 Prince Edward Island (P.E.I.) farms were examined for the presence of Shiga toxin-producing Escherichia coli (STEC) using a Vero cell assay (VCA). Multiple isolates from each positive sample were tested sim...
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The objectives of this study were to determine the carriage rate of Yersinia enterocolitica in the tonsils of slaughter hogs, and to characterize them with regard to phenotypic and virulence-associated properties. Of 202 pigs examined from an abattoir in Prince Edward Island, 85 were culture positiv...
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The plasmid profiles of oxytetracycline- and streptomycin-resistant isolates of Aeromonas salmonicida, Vibrio anguillarum, and Vibrio ordalii were examined by agarose gel electrophoresis. Bacterial isolates were from disease outbreaks in fish on the Atlantic and Pacific coasts. Resistant isolates we...
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Culture filtrates of 28 Salmonella enteritidis isolates were tested for toxicity on Vero-, CHO-, and human foreskin fibroblast (HFF) cells. Cytopathic effects on HFF cells were extensive, and were observed even with some filtrates diluted 1:256. Vero cells showed effects with filtrates diluted up to...
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Eighty-five blue mussels and 46 eastern oysters were collected from shellfish growing areas of 6 river systems in Prince Edward Island, and examined for aerobic and anaerobic bacterial flora. Animals were collected in different seasons during a 14-month period. Of a total of 907 isolates recovered, ...
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A microbiological assay was developed to quantify the concentrations of the quinolone antibiotics sarafloxacin and oxolinic acid in Atlantic salmon (Salmo salar) plasma. The assay was a modification of the Association of Official Analytical Chemists (AOAC) method for antibiotic concentrations in fee...
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27 consecutive isolates of Salmonella recovered from animals between March 1990 and October 1991 were tested for susceptibility to 17 antibiotics using a standard disc diffusion assay. The majority of isolates originated from bovine (44.4%), avian (29.6%) and equine (11.1%) sources. The most common ...
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Joints from 153 arthritic and 80 normal slaughter hogs were examined by culture for presence of bacteria. Although none of the normal joints yielded bacteria, 37% of the disease joints were positive for bacterial growth. Of 67 bacterial isolates obtained, 45% were Erysipelothrix rhusiopathiae. Occur...
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A total of 52 isolates of Escherichia coli belonging to enterotoxigenic serotypes from piglets with diarrhea were examined for hemolysis, production of cholera-like enterotoxin (LT) and susceptibility to 10 antimicrobial drugs. A strong association between production of LT and hemolysis was seen. Ni...
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The minimum inhibitory concentrations (MICs) of six antimicrobial agents were determined for Atlantic coast isolates of V. anguillarum, V. ordalii and A. salmonicida collected between 1985 and 1990. MIC values were calculated by agar dilution at 3 incubation temperatures (10, 20, 30 degrees C) for t...
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