Three Bubo virginianus each fed 5000 sporulated oocysts of Caryospora bubonis passed oocysts in their faeces after 11 to 13 days, peak oocyst production occurring between days 14 and 18. Three owls each fed 2 infected mice (dosed 4 weeks earlier with 25 000 C. bubonis oocysts) shed oocysts after 8 t...

Neoparamoeba pemaquidensis is an ubiquitous amphizoic marine protozoan and has been implicated as the causative agent for several diseases in marine organisms, most notably amoebic gill disease (AGD) in Atlantic salmon. Despite several reports on the pathology of AGD, relatively little is known abou...

A coccidian of the genus Pseudoklossia Leger and Duboscq, 1915 (Apicomplexa) occurs in the kidneys of abalone, Haliotis spp. (Mollusca), throughout waters off California. Parasites are often numerous in renal tissues, and developmental stages include oocysts, macrogametes, microgametocytes, and mero...

Eimeria bubonis n.sp. and Caryospora bubonis n.sp. both from Bubo virginianus from Saskatchewan, Canada, are described. E. bubonis differs from E. speotytoi and E. strigis in having shorter and narrower sporulated oocysts (22.8 plus or minus 2.7 mu m long and 21.7 plus or minus 2.4 mu m wide) with a...

In further laboratory studies in Canada [see preceding abstract], 12 h after infection 1st-stage larvae of Diplotriaena tricuspis had hatched, penetrated the gut wall, and entered the fat-body of the grasshopper Melanoplus sanguinipes (F.) and the locust Schistocerca gregaria (Forsk.). Capsule devel...

Three B. virginianus were each inoculated orally with 1.0 X 104 sporulated oocysts of E. bubonis and 3 were each fed 2 mice which had been previously intubated with sporulated oocysts of E. bubonis. In the directly infected owls the prepatent period was 5 to 6 days and the patent period was 4 and 6 ...

The American lobster fishery is a significant economic driver in coastal communities of North America. Increasingly, the impacts of infectious disease are recognized as important components and factors in the population ecology and subsequent management of the lobster fishery. Both environmental and...

Crows (Corvus brachyrhynchos) were experimentally infected with Diplotriaena tricuspis (50 to 530 larvae/bird) and killed at intervals from 4 to 291 days afterwards. The larvae caused traumatic lesions during their movements through the hepatic portal system. Giant cell granulomata surrounded dead n...

Sporocysts of Sarcocystis were recovered from the intestinal mucosa of A. acadicus from Yellowstone National Park, Wyoming, USA. Sporocysts measured 12.0 x 9.7 micro m (9.6-14.0 x 8.0-12.0 micro m; n = 100). Doses of 0, 500, and 2500 sporocysts were administered orally to 5 P. maniculatus and 5 Swis...

Lasalocid and pyrimethamine was administered to American lobsters infected with A. haemophila. Lasalocid was found to be toxic and acute mortality was observed, when administered in vivo in both infected and uninfected lobsters. Pyrimethamine was found very efficient to treat these ciliates at repea...

This study describes the sequential formation of lesions associated with the endogenous development of Sarcocystis rauschorum (Apicomplexa: Sarcocystidae) in varying lemmings, Dicrostonyx richardsoni. Lethal doses of sporocysts (greater than 500) were orally administered to lemmings examined 1-6 day...

Two cases of Amoeba-like infections in cultured warmwater marine fish are described, a systemic infection in pompano T. falcatus from Singapore and a gill infection in Mediterranean (Greece) sea bream S. auratus. All pompano showed marked systemic infection of Amoeba-like parasites in the gills, kid...

The potential for cross-transmission of Perkinsus karlssoni from bay scallops Argopecten irradians to various native Atlantic Canadian species of shellfish was investigated. Lateral proximal transmission was investigated whereby infected moribund bay scallops were maintained with uninfected individu...

Anophryoides haemophila is a ciliated protozoan and the causative agent of bumper car disease in lobsters. An in vitro system was developed to assess the effects of chemotherapeutants on ciliate motility and morphology. Monensin, formaldehyde and pyrimethamine + sulphaquinoxaline caused a dose-depen...

Lesions found in bay scallops Argopecten irradians imported into Atlantic Canada for culture were attributed to a parasite Perkinsus karlssoni. It appears, however, that stages from several organisms were combined to create the life cycle of P. karlssoni. The ultrastructure of the agent in lesions o...

A tetrazolium-based cytotoxicity assay was modified to determine the anti-protozoal activity of compounds towards the lobster parasite A. haemophila. To obtain reproducible results, A. haemophila maintained in continuous culture were preferable to freshly isolated A. haemophila. It was also found th...

A comparison of the coinfection of bay scallops with Perkinsus karlssoni and an as yet unidentified coccidian is described. P. karlssoni occurred in a range of host tissues including mantle, digestive gland, intestine, gill and gonad. Host tissue response elicited against P. karlssoni ranged from fo...

Isolation and purification of the trophozoite and schizont stages of the protozoan Perkinsus karlssoni , a parasite affecting Argopecten irradians , was achieved by macerating infected host tissue. Prior culture of infected tissue in fluid thioglycollate medium was unnecessary either for detecting P...

In North America, one of the largest traditional surviving fisheries involves the coldwater clawed American lobster Homarus americanus. However, post-harvest losses are conservatively estimated at 10-15%, representing an economic impact of $50-75 million annually. The mandate of the Lobster Health R...

The status of Perkinsus parasites continues to challenge scientists. Are they fungi or protistans? Are they apicomplexans? How many species of Perkinsus exist? Molecular biology is a powerful tool which has been used to address these questions of taxonomy and phylogeny. In particular, nucleotide seq...

Zygote development and oocyst wall formation of Eimeria truncata occurred in epithelial cells in renal tubules and ducts of experimentally infected lesser snow geese (Anser c. caerulescens). Post-fertilization stages were present throughout the kidneys beginning nine days post-inoculation. Initially...