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Sporozoites of Sarcocystis capracanis and S. tenella (Apicomplexa) penetrated all four cell types tested (bovine monocytes, BM; bovine pulmonary artery endothelial cells, CPA; Madin-Darby bovine kidney; and ovine monocytes). Sporozoites of S. tenella developed to meronts in BM and CPA; those of S. c...
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Meronts, merozoites, oocysts and unidentified stages of a new species of Eimeriidae were found in the reproductive tract of one of 4 C. canadensis nelsoni bulls shot at the beginning of the rut season in Saskatchewan, Canada. The eimeriids were seen free in the luminal content and, on one occasion o...
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The early endogenous development of S. rauschorum in D. richardsoni was studied. After determining the dose-response effects of sporocysts of S. rauschorum on lemmings (doses greater than 500 sporocysts were lethal during hepatic merogony), sporocysts were administered to additional lemmings that we...
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Coccidia-free, artificially reared lesser snow geese and ducks (Anas strepera, A. platyrhynchos and A. discors) were inoculated orally with sporulated oocysts of Eimeria sp. from the ureter of heavily infected juvenile lesser snow geese from Manitoba, Canada. In the experimentally infected geese, oo...
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Although 1993 landings of lobsters were valued at $300 million in Canada and $210 million in the United States, postharvest losses are 10-15% annually. "Bumper car" disease of lobsters caused by the scuticociliate Anophryoides haemophila, can be significant in coldwater impoundments. Altho...
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I. brachyrhynchi were recovered from faeces of experimentally infected juvenile crows (Corvus brachyrhynchos) and stored for up to 12 months in a 2.5% (w/v) solution of potassium dichromate at 4 degrees C. Sporocysts were released from the oocysts by grinding in a homogenizer. Various bile salts, ta...
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In May 1989 a previously unidentified apicomplexan parasite was found in brookstock bay scallops Argopecten irradians from the east coast of Canada. Host tissue responses ranged from focal hemocytic encapsulation to the formation of abscesses containing necrotic hemocytes, parasites and ceroid. Bay ...
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Developmental stages of Cryptosporidium sp. (Protozoa: Cryptosporiidae) were observed in tracheal epithelium of two groups of turkey poults from a farm in central Saskatchewan. Lesions associated with the parasite included excess mucus, epithelial hyperplasia and metaplasia and necrosis, and macroph...
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Between 1980 and 1982 the kidneys of 627 waterfowl from Saskatchewan, Canada, were examined for oocysts by a modified flotation technique. Eimeria spp. were detected in 136 of 289 ducks (11 of 12 species), 130 of 287 geese (2 species) and 2 of 4 whistling swans (Cygnus columbianus). Bucephala clangu...
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Besnoitia sp. was diagnosed in two caribou (Rangifer tarandus caribou) which died of pneumonia at the Assiniboine Park Zoo (Winnipeg, Manitoba, Canada) in 1983. During the following 3 yr besnoitiosis spread to an isolated herd of caribou, to mule deer (Odocoileus hemionus hemionus) and to reindeer (...
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Muscle containing macroscopic cysts of Sarcocystis sp. from naturally infected wild shoveler (Anas clypeata) ducks was fed to two captive striped skunks (Mephitis mephitis). The skunks passed sporocysts in their feces beginning 19 and 22 days post-infection, and continued to pass small numbers of sp...
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Sporulated oocysts (10.1 X 15.3 mu m) and free sporocysts (8.0 X 10.2 mu m, with a large, round sporocyst residuum consisting of a single refractile granule) of Sarcocystis campestris n.sp. were obtained from intestinal scrapings of Taxidea taxus (carcasses frozen 2 years at -20 deg C) from Saskatch...
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Crows were intubated with varying doses of sporulated oocysts of I. brachyrhynchi n.sp. from a naturally infected crow, and killed for necropsy at various hours postinoculation. Tissues were fixed in Bouin's fluid and processed for light microscopy. Sporulation of I. brachyrhynchi to typical isospor...
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Three Bubo virginianus each fed 5000 sporulated oocysts of Caryospora bubonis passed oocysts in their faeces after 11 to 13 days, peak oocyst production occurring between days 14 and 18. Three owls each fed 2 infected mice (dosed 4 weeks earlier with 25 000 C. bubonis oocysts) shed oocysts after 8 t...
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The etiological agent of 'bumper car' disease in lobsters Homarus americanus is described as a new species of ciliate, Anophryoides haemophila (Scuticociliatida: Orchitophryidae). A. haemophila n. sp. is distinguished from other species in the genus by the curved rectangular oral polykinetid 2, a so...
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A comparison of the coinfection of bay scallops with Perkinsus karlssoni and an as yet unidentified coccidian is described. P. karlssoni occurred in a range of host tissues including mantle, digestive gland, intestine, gill and gonad. Host tissue response elicited against P. karlssoni ranged from fo...
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Richardson's ground squirrels were infected with 1500 or 9000 sporocysts of Sarcocystis campestris from badgers. No lesions were found in animals killed one to three days postinfection (pi). Hepatitis and phlebitis of hepatic veins were present in animals killed between four and eight days pi. No me...
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Neoparamoeba pemaquidensis is an ubiquitous amphizoic marine protozoan and has been implicated as the causative agent for several diseases in marine organisms, most notably amoebic gill disease (AGD) in Atlantic salmon. Despite several reports on the pathology of AGD, relatively little is known abou...
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A coccidian of the genus Pseudoklossia Leger and Duboscq, 1915 (Apicomplexa) occurs in the kidneys of abalone, Haliotis spp. (Mollusca), throughout waters off California. Parasites are often numerous in renal tissues, and developmental stages include oocysts, macrogametes, microgametocytes, and mero...
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Isolation and purification of the trophozoite and schizont stages of the protozoan Perkinsus karlssoni , a parasite affecting Argopecten irradians , was achieved by macerating infected host tissue. Prior culture of infected tissue in fluid thioglycollate medium was unnecessary either for detecting P...