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Proteinase K was used for the cleaning of Sarcocystis cruzi (Apicomplexa) sporocysts prior to excystation. Bovine pulmonary endothelial cell cultures inoculated with the excysted sporozoites remained free of bacterial contamination for the duration of the experiment and had high yields of merozoites...
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The principal lesions due to the ciliate Anophryoides haemophila in experimentally infected lobsters are observed in the gills and connective tissue from the 9th week post-infection. These lesions were not related to the inoculum of parasites and were consistent in all inoculum studies (2000, 10 000...
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Mushroom tyrosinase (E.C. 1.14.18.1) was evaluated as a control material for use in a plasma prophenoloxidase assay in the American lobster Homarus americanus as part of the assay's validation procedure. Reconstituted. lyophilized tyrosinase solution was stable for 72 h at 2-4degreesC. Percent ...
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The purpose of this research was to investigate the salinity and formalin sensitivity of a ciliate parasite (Anophryoides haemophila) of the American lobster (Homarus americanus), and to examine the target-animal (lobster) safety of chemical-bath treatments involving low salinity, formalin, or chlor...
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Reference ranges for blood chemical and cellular components in the "healthy" population of interest are required to identify alterations in individuals in association with disease and disorders. The purpose of this study was to determine reference ranges for various constituents of hemolym...
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Chronic mortalities in lumpfish Cyclopterus lumpus reared in a saltwater recirculation system were associated with the presence of an intranuclear microsporidian. Morphological changes were characterized by infiltration of lymphocyte-like cells predominantly in the renal interstitium and also in the...
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The pathology caused by the ciliate Anophryoides haemophila was studied in experimentally infected lobsters. The first lesions due to the parasite were observed in gills and connective tissue from 9th week post-infection for the lower inocula and at week 4 for the 500,000 ciliate inoculum.. These le...
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Adult ticks were found on 2 cats and a dog in the Charlottetown area between June and November 1989. The 3 ticks were identified as I. dammini. Serum samples from the 2 cats were examined for antibodies to Borrelia burgdorferi; IFA titres were less than 1:32 in both cats. It is uncertain whether in ...
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Neoparamoeba pemaquidensis continues to be an ongoing problem for commercial finfish aquaculture and has also sporadically been associated with mass mortalities of commercially relevant marine invertebrates. Despite the ubiquity and importance of this amphizoic amoeba, our understanding of the biolo...
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ABSTRACT. Neoparamoeba pemaquidensis, the etiological agent of amoebic gill disease, has shown surprising sequence variability among different copies of the 18S ribosomal RNA gene within an isolate. This intra-genomic microheterogeneity was confirmed and extended to an analysis of the internal trans...
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This paper presents an overview of perkinsiosis in Salmo salar farmed in the Bay of Fundy, Canada, including information on the systematics of Perkinsus parasites, infection and disease caused by Perkinsus, lesions associated with the parasite in smolt and growout (market weight) salmon, differentia...
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Hematodinium is a parasitic dinoflagellate of numerous crustacean species, including the economically important Atlantic snow crab, Chionoecetes opilio. The parasite was isolated from C. opilio in the fall of 2010 and 2011 and cultured in vitro. Several media were tested, with Nephrops medium yieldi...
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American lobsters Homarus americanus were inoculated with a field isolate of the Gram-positive bacterium Aerococcus viridans var. homari, causative agent of gaffkemia, at 1 x 10(6), 1 x 10(4) or 1 x 10(2) colony forming units (CFU) kg(-1) or with sterile 3% NaCl and maintained at 10 or 15 degrees C ...
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We have performed a genomic characterization of a kinetoplastid protist living within the amoebozoan Neoparamoeba pemaquidensis. The genome of this "Ichthyobodo-related organism" was found to be unexpectedly large, with at least 11 chromosomes between 1.0 and 3.5 Mbp and a total genome size of at le...
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A combination of 16S rRNA sequencing and random amplified polymorphic DNA (RAPD) analysis was used to evaluate the genetic diversity within Aerococcus viridans var. homari, the causative agent of gaffkemia in lobsters. A collection of 7 A. viridans var. homari strains and 2 avirulent A. viridans-lik...
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Hemocytes of the American lobster (Homarus americanus H. Milne Edwards) were classified after examination of Wright-Giemsa stained cytocentrifuge preparations by brightfield light microscopy. Eleven hemocyte types were identified using morphologic criteria. The classification system was then used to...
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The Canadian lobster industry holds lobsters Homarus americanus in captivity for various periods to supply markets with live product year-round. Mortality during holding results in considerable losses, estimated at 10 to 15 % yr(-1) by the industry. This study examined the prevalence of Anophryoides...
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The concentration of arginine kinase (AK) in the hemolymph of lobsters may aid in the identification of muscle diseases in these animals in the same way that creatine kinase activity in blood plasma does in mammalian species. Both manual and automated analysis methods were developed for measuring AK...
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Potassium chloride (KCl: 330 mg/ml) was assessed as an euthanasia agent in American lobsters (Homarus americanus). Two groups of 10 lobsters (408.2 to 849.9 g) were maintained at 11.9 to 12.1 degrees C ('warm') and 1.5 to 2.5 degrees C ('cold') to evaluate the possible effect of ...
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Trichocyst morphology and development were explored using transmission electron microscopy in Hematodinium spp. isolated directly from Atlantic snow crab (Chionoecetes opilio) hemolymph and from in vitro cultures. Appearance of trichocysts defines the initiation of a morphological transition in the ...
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The objective of this study was to identify risk factors for productivity losses in the Canadian lobster industry. Lobster handling and fishing practices onboard 64 fishing boats and transportation conditions on 49 vehicles subsequently transporting these lobsters from fishing wharves to processing ...
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This study describes and compares lobster fishing and handling practices on various boats, transportation conditions between fishing wharfs and processing plants, and health assessments of lobsters followed from the time of harvest to the time of arrival at the processing plants during the spring an...
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