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The objective of this study was to identify risk factors for productivity losses in the Canadian lobster industry. Lobster handling and fishing practices onboard 64 fishing boats and transportation conditions on 49 vehicles subsequently transporting these lobsters from fishing wharves to processing ...
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This study describes and compares lobster fishing and handling practices on various boats, transportation conditions between fishing wharfs and processing plants, and health assessments of lobsters followed from the time of harvest to the time of arrival at the processing plants during the spring an...
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Two clones of 1.4 and 4.33 kilobase pairs (kbp) DNA inserts, were selected from a Sarcocystis cruzi sporozoite genomic library constructed in bacteriophage lambda gt10. These clones strongly hybridized with sporozoite and merozoite DNA and were evaluated as probes for detection of merozoite DNA in c...
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The scuticociliate Anophryoides haemophila, causes bumper car disease in American lobster (Homarus americanus) in commercial holding facilities in Atlantic Canada. While the parasite has been recognized since the 1970s and much has been learned about its biology, minimal molecular characterization e...
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Trichocyst morphology and development were explored using transmission electron microscopy in Hematodinium spp. isolated directly from Atlantic snow crab (Chionoecetes opilio) hemolymph and from in vitro cultures. Appearance of trichocysts defines the initiation of a morphological transition in the ...
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The Canadian lobster industry holds lobsters Homarus americanus in captivity for various periods to supply markets with live product year-round. Mortality during holding results in considerable losses, estimated at 10 to 15 % yr(-1) by the industry. This study examined the prevalence of Anophryoides...
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Studies were undertaken to monitor for the presence of Borrelia burgdorferi, the etiologic agent of Lyme disease, on Prince Edward Island, Canada. Gut contents were removed for culturing from seven engorged ticks collected in 1991-1992 including five Ixodes dammini (Spielman, Clifford, Piesman &...
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Hemocytes of the American lobster (Homarus americanus H. Milne Edwards) were classified after examination of Wright-Giemsa stained cytocentrifuge preparations by brightfield light microscopy. Eleven hemocyte types were identified using morphologic criteria. The classification system was then used to...
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A genomic library of Sarcocystis cruzi sporozoite DNA was constructed in bacteriophage lambda gt10. Recombinant phages containing insert DNA were selected by growth on Escherichia coli strain C600 hflA150. Of 14 clones examined, 11 contained DNA inserts ranging in size from approximately 1.45 kiloba...
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ABSTRACT. Neoparamoeba pemaquidensis, the etiological agent of amoebic gill disease, has shown surprising sequence variability among different copies of the 18S ribosomal RNA gene within an isolate. This intra-genomic microheterogeneity was confirmed and extended to an analysis of the internal trans...
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Visceral and systemic infection with a Dermocystidium -like organism was diagnosed recently in post-seawater entry smolts of Atlantic salmon farmed in the Bay of Fundy. In late summer and early fall, young fish developed "black smolt" disease, characterized by anorexia, lethargy and black skin. Para...
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This case report provides pathologic and confirmatory molecular characterization of an outbreak of bumper car disease caused by the scuticociliate Anophryoides haemophila in American lobster Homarus americanus, from a commercial holding facility in Nova Scotia, Canada. Although sporadically and anec...
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Sporozoites of S. hirsuta, although they also penetrated into bovine monocytes, developed to the schizogonous phase only in bovine pulmonary artery endothelial cells. Schizonts were evident from 14 days after sporozoite inoculation (DAI) and persisted to 62 DAI, when experiments were terminated. Mer...
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Spores identified as Myxobolus neurobius were found in head homogenates of both wild young Atlantic salmon Salmo salar and Arctic char Salvelinus alpinus . Formalin-preserved spores from Atlantic salmon measured an average of 12.9 x 9.4 mu m and contained two polar capsules that measured 6.4 x 2.9 m...
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The levels of production, ascarid burden and respiratory disease were measured on 15 purposively selected swine herds, and the relationships between the various measures of ascarid burden were examined. On each farm 30 randomly selected pigs were weighed and rectal fecal samples were collected at ap...
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Proteinase K was used for the cleaning of Sarcocystis cruzi (Apicomplexa) sporocysts prior to excystation. Bovine pulmonary endothelial cell cultures inoculated with the excysted sporozoites remained free of bacterial contamination for the duration of the experiment and had high yields of merozoites...
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The principal lesions due to the ciliate Anophryoides haemophila in experimentally infected lobsters are observed in the gills and connective tissue from the 9th week post-infection. These lesions were not related to the inoculum of parasites and were consistent in all inoculum studies (2000, 10 000...
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The concentration of arginine kinase (AK) in the hemolymph of lobsters may aid in the identification of muscle diseases in these animals in the same way that creatine kinase activity in blood plasma does in mammalian species. Both manual and automated analysis methods were developed for measuring AK...
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Mushroom tyrosinase (E.C. 1.14.18.1) was evaluated as a control material for use in a plasma prophenoloxidase assay in the American lobster Homarus americanus as part of the assay's validation procedure. Reconstituted. lyophilized tyrosinase solution was stable for 72 h at 2-4degreesC. Percent ...
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American lobsters Homarus americanus were inoculated with a field isolate of the Gram-positive bacterium Aerococcus viridans var. homari, causative agent of gaffkemia, at 1 x 10(6), 1 x 10(4) or 1 x 10(2) colony forming units (CFU) kg(-1) or with sterile 3% NaCl and maintained at 10 or 15 degrees C ...
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The purpose of this research was to investigate the salinity and formalin sensitivity of a ciliate parasite (Anophryoides haemophila) of the American lobster (Homarus americanus), and to examine the target-animal (lobster) safety of chemical-bath treatments involving low salinity, formalin, or chlor...