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We made a single intraperitoneal (IP) injection of morphine sulfate (40 mg/kg) into winter flounder and seawater acclimated rainbow trout at 10°C and then followed its disposition by measuring the change in plasma morphine concentration for 100 h using a morphine specific ELISA. Disposition also wa...
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An electron microscopic study of the red and white muscle fibres in the trunk musculature of the Kawakawa tuna ( Euthynnus affinis ) was carried out with a view to correlating their structure with metabolic adaptation. The red fibres which are considerably smaller in diameter (34.58 µm ± 6.16 ...
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Rainbow trout, Oncorhynchus mykiss, were injected with an adrenergic agonist. an adrenergic antagonist, or a cholinergic antagonist, via a dorsal aorta cannula. Spleen size and haemoglobin content were measured at rest and, for antagonists, following an air exposure stress. Injection of phentolamine...
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1. Mdx mice were used as a model for Duchenne muscular dystrophy; both lack dystrophin. It was hypothesized that the mdx condition would have a marked effect on the ability of diaphragm muscle from mdx mice to do active net work and generate power. This hypothesis was tested using the work-loop tech...
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The goal of our experiment was to elucidate the effect of stimulus duty cycle (the percentage of the cycle that the muscle was stimulated), phase (the relative timing of the imposed sinusoidal length change and stimulation), and muscle cycle frequency (the speed at which the muscle was cycled) on wo...
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Tuna are unusual among fish in that they are warm bodied. It is agreed that one adaptive advantage to being warm is that the warmth increases the rate of delivery of oxygen from the cell boundary to the mitochondria by myoglobin. Tuna have extremely high rates of oxygen uptake, much higher than othe...
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Eccentric contractions (activation during muscle lengthening) can cause muscle damage. The effect of phase of stimulation on the extent of muscle damage was studied by using the work-loop method. For the work-loop method, the muscle was subjected to sinus...
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Strips of isolated rat diaphragm muscle were attached to a servomotor–transducer apparatus, and the muscle length was cycled in a sinusoidal fashion about the length at which maximum isometric twitch force was developed, Lo. The amplitude of the length displacement (excursion ...
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In a recent paper Brown & Muir (1970) analysed the function of tuna gills from a theoretical viewpoint. Their analysis was based on Muir's work on the anatomy of tuna gills (Muir, 1969; Muir & Hughes, 1969; Muir & Kendall, 1968), and some assumptions on the physiology of tuna. In the present paper a...
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Pain is a major sympton in many medical conditions, and often interferes significantly with a person's quality of life. Although a priority topic in medical research for many years, there are still few analgesic drugs approved for clinical use. One reason is the lack of appropriate animal model...
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We examined the effects of midline and off midline incision locations, acid incisions closed with monofilament or silk suture on spontaneous swimming behaviour in fish. Each fish had a dummy transmitter surgically implanted into its peritoneal cavity. The behaviour of fish in each treatment was esti...
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Specific activity and kinetic constants of trypsin from the pyloric caeca of rainbow trout (Salmo gairdneri) were estimated. Although one group was fed more than twice as much as the other (1.8 compared with 0.7% body weight daily), there was no significant difference in the weight of the pyloric ca...
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Oxygen uptake from air and from water was measured during routine activity at 2TC in two faculta~iveair-breathing teleosts, iioplrrytl~rinus14 niraenintus and Etyrhrinlrs ~pt1trinsr.v.These fish brearhe air in hypuxic water. lsking a breath every I to 2 min. Gill ventilation increases in hypoxic wat...
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Most athletes stretch as part of their training regimen and it is commonly believed that this practice prevents muscle injury. We tested this belief using an animal model, in situ mouse extensor digitorum longus (EDL) muscle. One lower hindlimb was slowly stretched for 1 min on alternate days for 12...