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Effective methods of traceability are urgently required for use in research as well as in different types of aquaculture operations and to control trade in aquatic animals and products. In regard to the marking of fish, many different tagging methods have been described and the method to be used dep...
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Since its first description, the paramyxean parasite Marteilia refringens has been recognized as a significant pathogen of bivalve mollusks. The existence of a complex life cycle was postulated by many authors. Here we report the development of DNA-based detection assays as powerful tools to elucida...
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The protozoan parasite Perkinsus atlanticus (Azevedo, 1989) causes severe losses among cultured clams, Ruditapes decussatus. This parasite is routinely diagnosed by means of histology or incubation of gills in fluid thioglycollate medium. However, in order to develop models of experimental reproduct...
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The development of molecular diagnostic assays with increased sensitivity compared with conventional histological techniques is highly desirable for effective management of bonamiosis in cultured oyster stocks and wild populations. A real-time TaqMan PCR assay was developed for the specific detectio...
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The French mollusc production is mainly based on the Pacific cupped oyster, Crassostrea gigas. Since 1991, outbreaks of mass mortality of juveniles are reported during the summer period. These outbreaks are a major concern of oyster industry. Several studies have established given bacterial strains ...
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One of limiting factors in shrimp culture is the mortality at the larval stage. Often bacterial diseases are suspected to be the origin of this damage. This work concerns the study of relations between microorganisms (bacteria and Vibrio) and peneids. Observations induced a new approach to bacteriol...
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Microcell-type parasites of oysters are associated with a complex of diseases in different oyster species around the world. The etiological agents are protists of very small size that are very difficult to characterize taxonomically. Associated lesions may vary according to the host species, and the...
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A nested polymerase chain reaction (PCR) has been developed for the diagnosis of Marteilia refringens in mussels (Mytilus galloprovincialis) from Galicia (NW Spain). Specific primers were used in two consecutive amplification reactions, that were able to detect 15 fg of Marteilia DNA. The nested PCR...