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Immunohistochemical and ultrastructural methods were used to examine the distribution of elastin and the fine structure of the trabecular, nasal, branchial, and pericardial cartilages in the sea lamprey, Petromyzon marinus. The cells and matrix, as well as the overall organization of these component...
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1. Various tissues of the porcupine Hystrix hodgesoni including liver, intestine, stomach, spleen, kidney, brain and lung were examined for the presence of growth hormone binding sites. 2. Membranes were prepared from the aforementioned tissues and tested for binding to I-125-bovine growth hormone (...
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The goal of this study was to obtain a complete description of the elastic modulus and hysteresis of the mantle tissue of the squid Loligo pealei over the entire functional region of the mantle. In addition, in vivo strains were measured to allow. calculations of the amount of energy stored and retu...
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A collagenous extracellular matrix was previously considered to be a requirement for classification of true cartilage. Data from the lamprey and hagfish now clearly indicate that both of these jawless craniates have extensive non-collagenous, yet cartilaginous endoskeletons. Non-collagenous cartilag...
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Lampreys exhibit important structural and biochemical differences in their connective tissues relative to higher vertebrates. The lamprey ventral aorta wall, for example, is composed principally of microfibrils, not elastin and collagen as in higher vertebrates. Our working hypothesis was that this ...
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Lamprin, an insoluble non-collagen, non-elastin protein, is the major connective tissue component of the fibrillar extracellular matrix of lamprey annular cartilage. Here we demonstrate that the soluble monomer of lamprin is a family of highly hydrophobic, self-aggregating proteins with molecular ma...
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Thyroglobulin (TG) was localized in the endostyle of the anadromous sea lamprey, Petromyzon marinus L. by means of the unlabeled antibody peroxidase-antiperoxidase immunocytochemical method. TG was found localized on the apical surface and within the cytoplasm of type 2c and 3 cells and in some type...
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Immunoreactive fibronectin-like material was localized within tissues of agnathans (hagfishes and lampreys) by an immunoperoxidase technique. Fibronectin was detected in basement membranes and in loose and dense connective tissues throughout the agnathan body. A fibronectin-like component was also i...
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Marfan syndrome, a connective tissue disorder affecting the cardiovascular system, is caused by mutations of fibrillin-based microfibrils. These mutations often affect the calcium-binding domains, resulting in structural changes to the proteins. It is hypothesized that these Ca+2 binding sites regul...
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The physiological effects of repeated exposure to 9 mg l(-1) chloramine-T, a common aquaculture disinfectant for rainbow trout, were examined using a graded hypoxic challenge. Using an extracorporeal circulation, continuous measurements of blood PO2, PCO2 and pH were made and correlated with decreas...
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The morphology and the histochemistry of the mucus of the pleuronectid stomach were examined using light and electron microscopy. The stomach of the winter flounder (Pleuronectes americanus) and the yellowtail flounder (Pleuronectes ferruginea) possessed a glandular or cardiac region and an aglandul...
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The ontogeny and disease responses of Langerhans-like cells within lymphoid tissues of Atlantic salmon, Salmo salar, and rainbow trout, Oncorhynchus mykiss, were investigated. These cells were studied in situ with the use of two markers: the ultrastructural presence of Birbeck-like granules and immu...
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Infectious salmon anaemia (ISA) virus (ISAV) is a fish orthomyxovirus that has recently been assigned to the new genus Isavirus within the family Orthomyxoviridae. It possesses the major functional characteristics of the virus family including haemagglutinating, receptor destroying enzyme (RDE), and...
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