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A homogenous enzyme immunoassay (EIA) for measurement of serum thyroxine (T4) concentration was evaluated for use with canine and feline serum. The EIA method was linear from 0 to 150 nmol T4/L for human serum, 0 to 94 nmol T4/L for feline serum and 10 to 60 nmol T4/L for canine serum. Intra- and in...
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OBJECTIVE: To determine effects of preoperative administration of ketoprofen on whole blood platelet aggregation, buccal mucosal bleeding time, and hematologic indices in dogs after elective ovariohysterectomy. DESIGN: Randomized, masked clinical trial. ANIMALS: 22 healthy dogs. PROCEDURE: 60 minute...
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Hyperthyroidism can increase the renal excretion of magnesium and thus cause hypomagnesemia in various species. Anaerobically collected blood samples from 15 hyperthyroid and 40 normal, healthy cats were analyzed with an ion-selective electrode analyzer and a serum biochemical analyzer. There was no...
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Serum C-reactive protein (CRP) concentration was measured, using an automated immunoturbidimetric assay, in 44 clinically normal dogs and 67 dogs with band neutrophil count > or = 10(9) cells/L, and values were found to be significantly (P or = 10(9) band neutrophils/L resulted in a statistically...
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We used hydrogen peroxide to reproduce gill lesions typical of a broad assemblage of gill diseases encountered in aquaculture, and examined the degree of growth rate depression that it caused. Additionally, we compared growth rates of 60 gill-damaged fish when they were either kept separate from (n=...
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Tanks of healthy juvenile rainbow trout (Oncorhynchus mykiss) were exposed to chloramine-T (10 mg/litre; 40 fish) or formalin (200 mg/litre; 40 fish) for 1 h once per week for 4 weeks. The effect of this treatment on the primary stress response was evaluated by measuring circulating cortisol levels ...
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The concentration of arginine kinase (AK) in the hemolymph of lobsters may aid in the identification of muscle diseases in these animals in the same way that creatine kinase activity in blood plasma does in mammalian species. Both manual and automated analysis methods were developed for measuring AK...
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Mushroom tyrosinase (E.C. 1.14.18.1) was evaluated as a control material for use in a plasma prophenoloxidase assay in the American lobster Homarus americanus as part of the assay's validation procedure. Reconstituted. lyophilized tyrosinase solution was stable for 72 h at 2-4degreesC. Percent ...
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Cats with hyperthyroidism [(increased serum thyroxine (T(4))] commonly have increased serum alkaline phosphatase (ALP) activity in addition to other serum biochemical abnormalities. Serum biochemical profiles were obtained from 10 hyperthyroid cats which had increased serum ALP. Agarose gel electrop...
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American lobsters Homarus americanus were inoculated with a field isolate of the Gram-positive bacterium Aerococcus viridans var. homari, causative agent of gaffkemia, at 1 x 10(6), 1 x 10(4) or 1 x 10(2) colony forming units (CFU) kg(-1) or with sterile 3% NaCl and maintained at 10 or 15 degrees C ...
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The effect of oral treatment with natural or recombinant human interferon alpha (HIA) on inflammatory airway disease in young standardbreds was assessed in a double-blind, randomized clinical trial. A total of 34 horses with nasal discharge, excess mucus in the trachea, and a persistent cough of at ...
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The purpose of this research was to investigate the salinity and formalin sensitivity of a ciliate parasite (Anophryoides haemophila) of the American lobster (Homarus americanus), and to examine the target-animal (lobster) safety of chemical-bath treatments involving low salinity, formalin, or chlor...
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Serum sorbitol dehydrogenase (SDH) activities in 10 cows and nine horses were measured using an automated clinical analyzer. The serum samples were divided into aliquots that were stored at room temperature (21 degrees C), refrigerated (0-5 degrees C), or frozen (-30 degrees C). The stability of the...
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Reference ranges for blood chemical and cellular components in the "healthy" population of interest are required to identify alterations in individuals in association with disease and disorders. The purpose of this study was to determine reference ranges for various constituents of hemolym...
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This study was designed to investigate the effect of single short-duration bath-treatment with hydrogen peroxide (1000, 1250 or 1500 mg/l for 20 min), as currently used in treatment for sea lice, on the growth rate and gill morphology of rainbow trout. All three dose levels significantly reduced the...
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OBJECTIVE: To evaluate the effect of homologous amniotic fluid and meconium inoculated intratracheally into the lungs of neonatal rats. ANIMALS: 153 male 7-day-old Fischer-344 rats. PROCEDURE: Amniotic fluid was obtained by cesarean section from the uterus of pregnant rats and meconium was collected...
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During a longitudinal survey from June until October 1987 cytological blood parameters of smoltified marine cultured rainbow trout, Oncorhynchus mykiss (Walbaum), were monitored. In this period, the trout were exposed to environmental stress resulting in summer mortality. The statistical analyses sh...