Results

1 - 25 of 128
[Page generation failure. The bibliography processor requires a browser with Javascript enabled.]
Four avian reoviruses were orally inoculated into 1-day-old chickens to determine pathogenicity, virus persistence in the intestinal tract, and effects on body weight gains. Avian reoviruses Reo-25 and W3-492 belonged to two separate serotypes, and viruses TC 897 and W3-410 were antigenically relate...
[Page generation failure. The bibliography processor requires a browser with Javascript enabled.]
Two viruses, infectious salmon anaemia (ISA) virus and a novel togavirus-like virus, were isolated from ISA disease outbreaks that were first reported as a new syndrome, haemorrhagic kidney syndrome (HKS) affecting farmed Atlantic salmon Salmo salar L. on the East coast of Canada. Laboratory confirm...
[Page generation failure. The bibliography processor requires a browser with Javascript enabled.]
A novel in-vivo assay system for mapping and analyzing regulatory signals which promote transcription and expression of viral RNA genomes is described. The system was developed using infectious bursal disease virus (IBDV), a double-stranded RNA virus and Vero cells which are permissive for IBDV, as ...
[Page generation failure. The bibliography processor requires a browser with Javascript enabled.]
Because of the multiple-step process that is involved in the detection of mutagenized restriction enzyme sites in plasmid DNA, a simple and accurate method was developed to analyse the plasmid DNA of site-directed mutagenesis experiments from bacterial colonies. The desired mutated part is located b...
[Page generation failure. The bibliography processor requires a browser with Javascript enabled.]
[Page generation failure. The bibliography processor requires a browser with Javascript enabled.]
[Page generation failure. The bibliography processor requires a browser with Javascript enabled.]
Routine laboratory diagnosis of infectious salmon anaemia virus (ISAV) infection is primarily by reverse transcription polymerase chain reaction (RT-PCR) because of the high sensitivity and rapid turnaround time of the test. This paper describes methods for highly reproducible absolute viral load me...
[Page generation failure. The bibliography processor requires a browser with Javascript enabled.]
The lesions and etiologic agents associated with 13 outbreaks of respiratory disease in commercial chickens were investigated. Adenoviruses were isolated from tracheal and lung tissues of affected chickens in all 13 outbreaks. Escherichia coli was isolated from the lung of an occasional bird. The tr...
[Page generation failure. The bibliography processor requires a browser with Javascript enabled.]
A primer pair was designed from the published nucleotide sequence of the coding region of the bovine herpesvirus 1 (BHV-1) thymidine kinase (tk) gene for use in detection of the virus by use of polymerase chain reaction (PCR) amplification of 12 BHV-1 strains (3 ATCC and 9 local isolates). A tk dele...
[Page generation failure. The bibliography processor requires a browser with Javascript enabled.]
Infectious salmon anaemia virus (ISAV), a member of the family Orthomyxoviridae, is an important fish pathogen in marine aquaculture in the Northern Hemisphere. The pathogenesis of ISAV infection at the cellular level is not well studied. We investigated three permissive cell lines for ISAV, (SHK-1,...
[Page generation failure. The bibliography processor requires a browser with Javascript enabled.]
Infectious salmon anaemia virus (ISAV), a pathogen in marine aquaculture, belongs to the genus Isavirus, family Orthomyxoviridae. There is limited information on how ISAV interacts with host defences. To study ISAV-antibody interactions, virus neutralization (VN) assays were performed in the cell li...
[Page generation failure. The bibliography processor requires a browser with Javascript enabled.]
Infectious salmon anaemia virus (ISAV), an orthomyxovirus-like virus, is an important fish pathogen in marine aquaculture. Virus neutralization of 24 ISAV isolates in the TO cell line using rabbit antisera to the whole virus and comparative sequence analysis of their haemagglutinin (HA) genes have a...
[Page generation failure. The bibliography processor requires a browser with Javascript enabled.]
As nonenveloped viruses, the aquareoviruses and orthoreoviruses are unusual in their ability to induce cell-cell fusion and syncytium formation. While an extraordinary family of fusion-associated small transmembrane (FAST) proteins is responsible for orthoreovirus syncytiogenesis, the basis for aqua...
[Page generation failure. The bibliography processor requires a browser with Javascript enabled.]
Several outbreaks of clinical tenosynovitis in poultry were investigated. Staphylococcus aureus was recovered from tendon tissue of from 52.98% of affected chickens in different outbreaks. It was also present in the liver and heart blood, and seemed to contribute to the high mortality observed follo...
[Page generation failure. The bibliography processor requires a browser with Javascript enabled.]
[Page generation failure. The bibliography processor requires a browser with Javascript enabled.]
[Page generation failure. The bibliography processor requires a browser with Javascript enabled.]
[Page generation failure. The bibliography processor requires a browser with Javascript enabled.]
Non-typhoid Salmonella serovars remain a potential threat to human health, and beef cattle and broiler chickens are possible sources of these organisms on Prince Edward Island (PEI). In this study, the ceca of beef cattle belonging to fasted and non-fasted groups, and broiler chickens were examined ...
[Page generation failure. The bibliography processor requires a browser with Javascript enabled.]
BACKGROUND: Infectious salmon anaemia (ISA) virus (ISAV), an important pathogen of fish that causes disease accompanied by high mortality in marine-farmed Atlantic salmon, is the only species in the genus Isavirus, one of the five genera of the Orthomyxoviridae family. The Isavirus genome consists o...
[Page generation failure. The bibliography processor requires a browser with Javascript enabled.]
A total of 20 hybridoma cell lines secreting monoclonal antibodies (MAbs) against E. coli expressed bovine herpesvirus-1 (BHV-1) gD fusion protein were produced following the fusion of Sp2/0 myeloma cells with splenocytes from BALB/c mice immunized previously with immunoaffinity purified BHV-1 gD fu...
[Page generation failure. The bibliography processor requires a browser with Javascript enabled.]
Mast cells are crucial effector cells in host defense and immunoregulation. They are equipped with an impressive array of receptor systems which allows them to rapidly respond to pathogen-associated signals. G protein-coupled receptors (GPCRs) mediate mast cell activation and mediator release in res...
[Page generation failure. The bibliography processor requires a browser with Javascript enabled.]
Experiments were undertaken to examine the chymotrypsin sensitivity and trypsin sensitivity of 13 avian reoviruses, and to determine if there was any correlation with pathogenicity of some chicken reoviruses. A wide variation in the degree of sensitivity of avian reoviruses to chymotrypsin and tryps...
[Page generation failure. The bibliography processor requires a browser with Javascript enabled.]
Bovine semen samples spiked with bovine herpesvirus 1 (BHV-1) were used to compare dot blot hybridization, polymerase chain reaction (PCR), and virus isolation for detection of BHV-1 in bovine semen. The PCR amplification used primers targeting the BHV-1 thymidine kinase gene and a nucleic acid rele...
[Page generation failure. The bibliography processor requires a browser with Javascript enabled.]
14 avian reovirus isolates adapted to replicate in an African green monkey (Vero) cell line were studied for the nature of their replication. The growth curves of 5 viruses showed them to be highly cell-associated in Vero cells. Different procedures were examined for releasing the cell-associated vi...
[Page generation failure. The bibliography processor requires a browser with Javascript enabled.]
Finfish nodaviruses (betanodaviruses) can cause highly destructive infections in numerous species of farmed marine fish larvae and juveniles worldwide. The betanodavirus genome consists of two single-stranded positive-sense RNA molecules (RNA1 and RNA2). The virus can be classified into four genotyp...