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A method for obtaining algal chromosomal preparations is described employing the Fuelgen method for DNA staining, Fe-propionocarmine as an enhancing stain, and cupra-ammonium to remove cell wall material. Fe-propionocarmine applied as a gradient to the slide provides cells stained with the Feulgen s...
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The vertical zonations of organisms on rocky shores are described for 2 sites. The principal study area, near North Rustico, is in the Prince Edward Island[PEI] National Park. The general pattern at this site was a zone of blue-green algae at the highest level in the intertidal zone; a zone of Fucus...
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The construction and use of pottery units designed for algal culture studies involving the transplanting of laboratory initiated stock is examined. Several types are described, either for direct attachment to shore rock or supporting vehicles. Pottery units are inexpensive, simple to construct, dura...
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A method is described employing a primary pottery unit for seeding and growing C. crispis Stackhouse and a secondary concrete platform for support and protection. The combined units facilitate long-term studies where frequent removal of plants for observation is required and where field conditions a...
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Filament and gamete morphology, mating tests and hybridizations reveal the presence of eight taxa of Urospora from the Pacific and Atlantic coasts of North America. Three are dioecious and anisogamous: U. penicilliformis, U. neglecta and U. wormskioldii (synonym: U. vancouveriana). A fourth, U. bang...
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We cultured and sequenced newly collected material of Percursaria dawsonii Hollenberg et I. A. Abbott, a poorly known epibiont of limpets found along the west coast of North America. Zoospores, parthenogametes, and zygotes exhibited empty-spore germination, which produced a prostrate system that exp...
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N. ulvacea (Berkeley ex Kuetz.) PT Cleve, a marine colonial blade-forming diatom originally discovered in Scotland, was collected at North Rustico, Prince Edward Island, Canada, during the winter of 1971. It formed dense stands of Porphyra-like fronds, ranging up to 60 mm long .times. 20 mm wide. Th...
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P. meridionalis and R. constricta overlap in dense, almost pure stands at Porlier Pass, Galiano Island, British Columbia [Canada]. Clones, 10 of each, established from single spores, reproduced true to type through 11 generations spread over a 6 yr period. Prasiola and Rosenvingiella probably are di...
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Amplification of the ribosomal cistron of Urospora isolates from within the 18S ribosomal RNA (rRNA) gene through the internal transcribed spacer (ITS) and 5.8S regions into the 28S rRNA gene produced fragments of varying lengths. The lengths depended on the absence or presence of up to three group ...
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An outbreak of food poisoning in Canada during autumn 1987 was traced to cultured blue mussels (Mytilus edulis ) from the Cardigan Bay region of eastern Prince Edward Island (P.E.I.). The toxin, identified as domoic acid, had not previously been found in any shellfish and this outbreak represents th...
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The toxin-producing diatom Nitzschia pungens Grunow forma multiseries Hasle from three toxic blooms in two Prince Edward Island estuaries, spanning 1987-89, was studied using light and electron microscopy. Cell ultrastructure of N. pungens is, in general, similar to that of other species of Nitzschi...