Calves were inoculated intratracheally with 5 X 10(7), 5 X 10(8), or 5 X 10(9) colony forming units of either 18-hour stationary phase cultures or 4-hour log phase cultures of Pasteurella haemolytica. The log phase culture at all concentrations produced more severe clinical signs, hematological chan...

Bovine alveolar macrophages cultured for 12 hours with normal bovine bronchoalveolar washing supernatants (BWS) showed impaired phagocytosis of Pasteurella haemolytica when compared with phagocytosis in control cultures. Such impairment was not observed when another gram-negative bacillus, Yersinia ...

Liposomes were prepared from phosphotidylcholine and cholesterol at a molar ratio of 4:1. One group of 10 fish received an intraperitoneal injection of multilamella liposomes (MLV), while another group received large unilamellar liposomes (LUV). Approx. 20 micro Ci of tritiated cholesterol had been ...

In 3 experiments, calves were vaccinated by intrabronchial or subcutaneous injection of formalinized Pasteurella haemolytica. Immunization was evaluated by assessing the clinical and pathologic responses to pulmonary challenge exposure with live homologous bacteria. Serum and bronchoalveolar washing...

Water extracts of virulent Brucella abortus were able to inhibit phagosome-lysosome fusion in unelicited murine peritoneal macrophages following ingestion of yeast. Extracts from an avirulent strain were unable to produce a similar effect. Lipopolysaccharide from B abortus did not appear to be invol...

A 16-month seroepizootiologic study of bovine respiratory syncytial virus (BRSV) infection was conducted in a dairy herd. Results indicated that antibodies to BRSV present in serum from newborn calves were derived through the ingestion of colostrum. This passive immunity in calves became undetectabl...

Transformation of peripheral blood lymphocytes from pony foals vaccinated and subsequently infected with Corynebacterium equi was studied. Three foals were vaccinated on two occasions using a formalinized C. equi vaccine with aluminum hydroxide as an adjuvant. Three nonvaccinated foals served as con...

Pasteurella haemolytica was found to have a cytotoxic effect on the peripheral blood mononuclear leukocytes of cattle, sheep, and goats, but no effect on the mononuclear leukocytes of swine, horses, or humans. In contrast, Escherichia coli caused marked cell death among mononuclear leukocytes of all...

Cultured bovine alveolar macrophages phagocytosed formalized 125I-labeled Pasteurella haemolytica organisms poorly when compared with uptake of an identical number of radiolabeled Yersinia enterocolitica organisms. The presence of immune serum enhanced uptake of P haemolytica, yet at high bacteria-t...

Topics discussed in this review include classification of viruses, virus structure, epidemiology of viral infections, cell-mediated and humoral response, viral pathogenicity, viral defences and the detection, treatment and prevention of viral diseases..

Pasteurella haemolytica exerted a cytotoxic effect on bovine polymorphonuclear neutrophil leukocytes. This effect was less than that seen with cultured alveolar macrophages or peripheral blood monocytes. When alveolar macrophages were cultured with Pasteurella haemolytica, macrophages produced less ...

Six foals were inoculated intrabronchially with a suspension of Corynebacterium equi. Six weeks before this challenge, three foals were vaccinated with a C. equi bacterin. Three foals were unvaccinated controls. All foals developed a severe bronchopneumonia in the inoculated lung, indicating that va...

Intradermal injection of phytohemagglutinin was used to evaluate the integrity of cell-mediated immunological reactions in Doberman puppies thought to be predisposed to demodicosis. Results indicate a statistically significant deficiency of cutaneous delayed response in these dogs when compared with...

The in vitro mitogen response of whole blood turkey lymphocytes to various concentrations of steroid hormones was evaluated. Corticosterone (COS) at concentrations between 1 and 80 ng/ml significantly suppressed the proliferative response (3H-thymidine incorporation) to phytohemagglutinin (PHA) and ...

Cattle submitted to the University of Minnesota for surgical correction of left displaced abomasum (LDA) were examined for the in vitro phagocytic and bactericidal activities of their polymorphonuclear leukocytes (PMN). The PMN from cattle with LDA with or without concurrent infection had depressed ...

Seminal plasma was determined to have immunosuppressive activities in vitro on Concanavalin A-induced blastogenesis of bovine lymphocytes. This immunosuppressive activity was isolated and characterized using a pool of seminal plasma from five bulls. The immunosuppressive activity was associated with...

From day 57 of pregnancy, 12 Yorkshire gilts were given a diet without and with 5% rapeseed oil, supplemented with Se 0.1 mg and DL- alpha -tocopherol (as tocopherol acetate) 22 IU/kg feed. Colostrum (day 0) and milk (days 7, 14, 21 and 28 post partum) were sampled from gilts. At farrowing, 3 piglet...

Forty calves were used to evaluate the immune response effects induced by a wide range of dietary vitamin A intake levels. The immune response was not affected by the tested doses of vitamin A administered orally during a 56 day period. There was no change in plasma immunoglobulin concentrations and...

The intragastric inoculation of a suspension of Corynebacterium equi on five consecutive days induced severe ulcerative colitis, typhlitis, and lymphadenitis of colonic and cecal nodes in two ponies necropsied three weeks after infection. No gross lesions were observed in two ponies necropsied ten d...

During a 19-month period, the agar gel immunodiffusion (AGID) test was used as an aid in differential diagnosis of paratuberculosis in 51 cattle with a history of chronic weight loss and/or chronic diarrhea. Thirty-three cattle were AGID test-positive for paratuberculosis. Twenty-eight cattle (87.5%...

Six foals were inoculated intrabronchially with a suspension of C. equi. Six weeks before this challenge, three foals were vaccinated with a C. equi bacterin. Three foals were unvaccinated controls. All foals developed a severe bronchopneumonia in the inoculated lung, indicating that vaccination was...

The induction of protection against xenoma development by vaccinated (previously exposed to a low-virulence strain of Loma salmonae; n=40) and naive (n=40) rainbow trout (Oncorhynchus mykiss) challenged with the virulent typical strain of L. salmonae, was determined. Prevalence of infection was lowe...