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The induction of protection against xenoma development by vaccinated (previously exposed to a low-virulence strain of Loma salmonae; n=40) and naive (n=40) rainbow trout (Oncorhynchus mykiss) challenged with the virulent typical strain of L. salmonae, was determined. Prevalence of infection was lowe...
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An immunofluorescent antibody test (IFAT) developed for the diagnosis for plasmacytoid leukemia was evaluated against histology under field conditions. Previously published results from a laboratory evaluation indicated that the IFAT had a much higher sensitivity than did histology. One hundred seve...
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The meconium aspiration syndrome is an important cause of respiratory distress in newborn infants. Alveolar macrophages (AMs) provide a first line of defense in the lower respiratory tract against inhaled pathogens and particles such as meconium. In this study, we examined the effect of meconium on ...
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A lymphocyte stimulation test using antigens of Corynebacterium equi was used to compare the response of peripheral blood lymphocytes from foals with C equi pneumonia with those of clinically normal foals and adult horses. The test clearly distinguished infected foals from normal foals when tested i...
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Temperatures above 20 degrees C or below 9 degrees C interrupt the life cycle of the gill intracellular microsporidian parasite L. salmonae prior to sporogony, inhibiting the production of xenomas. This study intended to characterize this life-cycle failure. Juvenile rainbow trout were experimentall...
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A genomic library of Sarcocystis cruzi sporozoite DNA was constructed in bacteriophage lambda gt10. Recombinant phages containing insert DNA were selected by growth on Escherichia coli strain C600 hflA150. Of 14 clones examined, 11 contained DNA inserts ranging in size from approximately 1.45 kiloba...
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The present study describes culture, virulence and antigenic characteristics of a Rickettsiales-like organism (RLO) associated with mortality in farmed Atlantic salmon in eastern Canada. Clinical disease was reproduced in naive Atlantic salmon parr by intraperitoneal i.p. inoculation with kidney hom...
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The ontogeny of major histocompatibility class II antigens in small intestine enterocytes of postnatal C3H/He mice was investigated. Cryosections of duodenal, jejunal, and ileal segments from 7-, 14-, 16-, 20-, 21-, 23-, 25-, 27-, 28-day-old and 7-week-old mice were stained for the class II antigens...
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The qualitative and quantitative expression of major histocompatibility class II antigens was investigated in the absorptive epithelium of the duodenum, jejunum, and ileum from mice of C3H/He (H-2k haplotype) and C57BL/6 (H-2b haplotype) strains by peroxidase-antiperoxidase labelling and image analy...
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In determining the effective vaccine spore dose of a low-virulence strain of Loma salmonae to limit micro-sporidial gill disease in trout, we found that fish receiving 10(3) to 10(5) killed spores had the best protection against experimental infection, with 85% fewer xenomas in their gills than in t...
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Resistance to re-infection of rainbow trout to Loma salmonae, a microsporidian gill parasite has been previously documented and this study examined how rapidly this resistance develops. Naive rainbow trout were inoculated intraperitoneally (IP) with an inactivated spore-based vaccine and were then g...
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The early ultrastructural stages of Loma salmonae were studied in the gills of experimentally infected rainbow trout. No parasitic stages were identified during the first 2 wk of the infection. By week 3 postexposure (PE), uninucleate and binucleate meronts were recognized within host cells (no xeno...
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The objectives of the research were to compare the efficacy of ProVale yeast beta-glucan (beta-glucan) with that of a previously tested research-grade yeast beta-glucan preparation when administered as an intraperitoneal (IP) injection and to also pilot test the effectiveness of ProVale yeast beta-g...
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Serum C-reactive protein (CRP) concentration was measured, using an automated immunoturbidimetric assay, in 44 clinically normal dogs and 67 dogs with band neutrophil count > or = 10(9) cells/L, and values were found to be significantly (P or = 10(9) band neutrophils/L resulted in a statistically...
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Loma salmonae, a microsporidian parasite of salmonids of the genus Oncorhynchus, is a significant cause of economic loss in pen-reared chinook salmon (O. tschawytscha). Final stages of L. salmonae infections are easily recognized by the xenomas that form in the gills during sporogony. However, early...
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Sporozoites of S. hirsuta, although they also penetrated into bovine monocytes, developed to the schizogonous phase only in bovine pulmonary artery endothelial cells. Schizonts were evident from 14 days after sporozoite inoculation (DAI) and persisted to 62 DAI, when experiments were terminated. Mer...
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The objective of this study was to determine the agreement between ELISA tests conducted using three O. ostertagia antigens: crude adult worm, larval stage 4 (L4) excretory/secretory (ES) and adult ES. This study was carried out on 289 Holstein cows from five herds in Prince Edward Island and one he...
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Although the mollusc immune system has been studied at the cellular level, the response to pathogens at gene expression level has not been thoroughly investigated. This study aimed to investigate the early molecular response of hemocytes of soft-shell clams, Mya arenaria, to Vibrio splendidus strain...
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Experimental infection of rainbow trout juveniles with L. salmonae at a water temperature of 15 degrees C yielded detectable parasite DNA within the gills by the second week after exposure (AE) and detectable spore-wall antigen within developing xenomas by week 3 AE, as determined by in situ hybridi...
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Angiostrongylus vasorum, French Heartworm, is a metastrongylid nematode infecting the pulmonary arteries and right heart of wild and domestic canids in various regions of the world. Infection in dogs can result in fatal cardiopulmonary disease. A single endemic focus of A. vasorum in North America o...
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