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A new type of soyabean, AC Proteus, containing higher protein than conventional cultivars was evaluated using Landrace x Hampshire pigs, weighing about 10 kg. In the first trial, AC Proteus was included at 0, 4, 8, 12, 16 or 20% of the isonitrogenous and isoenergetic soyabean meal-based diets and fe...
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Effects of feeding heat-treated canola (C), soybean (S) and flax (F) or mixtures on growth and slaughter characteristics, taste and fatty acid (FA) composition of beef tissue were investigated using 128 crossbred steers to determine the potential of improving the nutritional quality of beef for huma...
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Two feeding trials were conducted to evaluate lupin seed (Lupinus albus) as a replacement for soyabean meal at levels of 0, 5, 10, 15, 20 and 25% of starter diets fed to 192 pigs, from 10 to 20 kg liveweight. ADF and NDF concentrations in the diets increased as the proportion of lupin in the diet in...
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A major problem for the mussel aquaculture industry on Prince Edward Island, Canada surfaced in the late 1990s with the introduction of a fouling organism commonly known as the clubbed tunicate, Styela clava. This organism has the ability to infest mussel cultivation equipment to the point where pro...
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The welfare consequences of long-distance transportation of animals remain a controversial topic. Animals that stand for most of the long journey (especially if additional muscular activity is required to deal with postural instability) are at risk of developing fatigue. Previous observational studi...
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This study investigated effects of roasted or extruded oilseed supplementation ranging in n-6/n-3 ratios from 0.3 to 5.0 on the fatty acid composition and expression of delta-5 desaturase (Δ5d) and Δ6-desaturase (Δ6d) protein in commercial steer cheek (m. masseter) and diaphragm (pars costalis di...
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In two trials, Arctic char (Salvelinus alpinus) semen was frozen in 0.5 mL straws using extenders consisting of 0.3 M glucose and 10%, 12.5% or 15% methanol. Cryopreserved semen was thawed by immersing straws in 25°C water for 17 s (11.6°C s-1) or in 5°C water for 60 s (3.3°C s-1). The viability...
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Lower fertility of preserved stallion semen may be caused by damage to the spermatozoa or premature capacitation during storage. We investigated the use of the flavonoid, quercetin, with a standard skim milk extender for storage of stallion spermatozoa to prevent premature capacitation and acrosome ...
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The effects of dietary lipid from 4 experimental diets on the fatty acid composition and cholesterol content of spermatozoa and their plasma membranes and their consequences for sperm viability after cryopreservation were evaluated in rainbow trout. The 4 sources of lipid were herring oil (adequate ...
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This study investigated the relationship between fertility and fatty acid (FA) profiles of egg chorions, total egg, and sperm cells of Arctic char, Salvelinus alpinus (L.) broodstock housed in tanks and fed a commercial diet. Fertility of semen from individual males was assessed using pooled eggs, a...
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Seminal plasma protein of Arctic char Salvelinus alpinus was. characterized using sodium dodecyl sulfate (SDS) gel electrophoresis. Twelve protein bands with molecular weights of 7.2, 12.4, 15.3, 20.0, 20.4, 22.6, 39.4, 66.3, 74.0, 92.0, 94.5, and 130.1 kilodaltons (kDa) were detected. The effect of...
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The total fatty acid composition of rainbow trout (Oncorhynchus mykiss) spermatozoa and seminal plasma was investigated in fresh and in stored semen using gas liquid chromatography. Triglycerides, phospholipids, and cholesterol were measured and the effect of fatty acids, triglycerides, and choleste...
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The objective of this study was to maintain the viability of chilled rainbow trout (Oncorhynchus mykiss) eyed eggs during storage using oxygenated perfluorochemical (PFC). Three trials were conducted using eggs at 161, 180 or 217 degree days (days from fertilization x incubation temperature in degre...
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Cryopreservation of Arctic charr semen was investigated using 3 diluents (0.3 M glucose, 0.3 M glucose with 0.011 M KCl and 0.137 M NaCl, 0.011 M KCl, 0.004 M Na2HPO4 with 7.5 g/litre L- alpha -lecithin adjusted to pH 7.5) 3 cryoprotectants [10% dimethyl sulphoxide (DMSO), 10% dimethylacetamide (DMA...
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Mussel aquaculture on Prince Edward Island (PEI), Canada, is an important but relatively new industry. Although seed manipulation using hatcheries for mussel culture occurs on the west coast of North America, seed supply on the east coast of Canada, including Prince Edward Island (PEI), is based sol...
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Several media for the storage of fresh semen from rainbow trout at 4 degrees C were evaluated. Semen was diluted with an equal volume of fluorocarbon, a 1:1 fluorocarbon:extender emulsion, a 2:1 fluorocarbon:extender emulsion, or with extender, or was stored undiluted. The semen was stored under moi...
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Egg quality in Arctic char, Salvelinus alpinus, kept indoors at 7 °C during spawning season was morphologically classified. Four egg categories based on lipid droplet distributions and egg diameter were characterized. Eggs with homogenous lipid vesicle distributions and a uniform size were classif...
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Fifteen extenders were produced by adding dimethyl sulfoxide (DMSO) at 8, 10 or 12% of diluent volume to 5 diluents. All extenders were cooled to 4 degrees C. Pooled Atlantic salmon (Salmo salar ) semen with greater than 90% progressive motility was kept at 4 degrees C and added to each extender so ...