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Semen from [male] fowls of a randombred line and a line selected for increased fertility duration of thawed semen (12 [male][male] per line) was diluted in Lake's diluent with or without glycerol. Half of each sample was frozen to -196 degrees C at -6 degrees per min, and thawed next morning. All se...
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The effect on nutritional quality of barley, maize, oats and wheat after flame roasting at 3 temperatures for each cereal (between 74 and 193 degrees C) in a commercial propane heated roaster at 67, 100 and 133% of the manufacturer's suggested temperatures was studied. A sample of unroasted material...
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120 broiler-type [male][male] were kept under a regime of 23-h light:1-h darkness (23L:1D) at 54 lux from 1 day to 5 wk of age, when they were divided into 6 groups subjected to 12L:12D or 16L:8D at 5.4, 10.8 or 54 lux. Semen production and the fertility of semen diluted 1:3 in Lake's diluent were s...
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Lower fertility of preserved stallion semen may be caused by damage to the spermatozoa or premature capacitation during storage. We investigated the use of the flavonoid, quercetin, with a standard skim milk extender for storage of stallion spermatozoa to prevent premature capacitation and acrosome ...
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The effects of dietary lipid from 4 experimental diets on the fatty acid composition and cholesterol content of spermatozoa and their plasma membranes and their consequences for sperm viability after cryopreservation were evaluated in rainbow trout. The 4 sources of lipid were herring oil (adequate ...
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Cryopreservation of Arctic charr semen was investigated using 3 diluents (0.3 M glucose, 0.3 M glucose with 0.011 M KCl and 0.137 M NaCl, 0.011 M KCl, 0.004 M Na2HPO4 with 7.5 g/litre L- alpha -lecithin adjusted to pH 7.5) 3 cryoprotectants [10% dimethyl sulphoxide (DMSO), 10% dimethylacetamide (DMA...
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Mussel aquaculture on Prince Edward Island (PEI), Canada, is an important but relatively new industry. Although seed manipulation using hatcheries for mussel culture occurs on the west coast of North America, seed supply on the east coast of Canada, including Prince Edward Island (PEI), is based sol...
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Several media for the storage of fresh semen from rainbow trout at 4 degrees C were evaluated. Semen was diluted with an equal volume of fluorocarbon, a 1:1 fluorocarbon:extender emulsion, a 2:1 fluorocarbon:extender emulsion, or with extender, or was stored undiluted. The semen was stored under moi...
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Fifteen extenders were produced by adding dimethyl sulfoxide (DMSO) at 8, 10 or 12% of diluent volume to 5 diluents. All extenders were cooled to 4 degrees C. Pooled Atlantic salmon (Salmo salar ) semen with greater than 90% progressive motility was kept at 4 degrees C and added to each extender so ...
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The objective was to determine the oxidative stability of Arctic char (Salvelinus alpinus) semen following dietary supplementation with lowbush blueberry (Vaccinium angustifolium) product, alpha-tocopherol, alpha-tocopherol+blueberry product, or alpha-tocopherol+astaxanthin. Sperm lipid peroxidation...
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The accuracy of three in vitro methods for estimating the proportion of dead rainbow trout (Onchorhynchus mykiss ) spermatozoa was investigated. Motility rating, fluorometry using ethidium bromide, and lactate dehydrogenase (LDH) activity in seminal plasma were compared. Semen samples were prepared ...
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Successful preservation of stallion spermatozoa is difficult because sperm damage occurs from lipid peroxidation during storage. We investigated the use of antioxidants with a standard skim milk extender and several gas environments during storage of stallion spermatozoa to reduce the harmful effect...
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Although dramatic improvements in growth rates have been documented in growth-enhanced transgenic salmonid fish, prior to commercial implementation of this technology, there is a need for further information relating to the physiology of a number of commercially important production traits. In 1996 ...
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In two trials, Arctic char (Salvelinus alpinus) semen was frozen in 0.5 mL straws using extenders consisting of 0.3 M glucose and 10%, 12.5% or 15% methanol. Cryopreserved semen was thawed by immersing straws in 25°C water for 17 s (11.6°C s-1) or in 5°C water for 60 s (3.3°C s-1). The viability...
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This study investigated the relationship between fertility and fatty acid (FA) profiles of egg chorions, total egg, and sperm cells of Arctic char, Salvelinus alpinus (L.) broodstock housed in tanks and fed a commercial diet. Fertility of semen from individual males was assessed using pooled eggs, a...
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Egg quality in Arctic char, Salvelinus alpinus, kept indoors at 7 °C during spawning season was morphologically classified. Four egg categories based on lipid droplet distributions and egg diameter were characterized. Eggs with homogenous lipid vesicle distributions and a uniform size were classif...