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Nine multiparous cows in early lactation were fed alfalfa silage ad libitum twice daily, a grain-based concentrate five times daily, and one of three protein sources five times daily. Supplemental proteins were soya-bean meal, raw coarse-ground sweet white lupins or roasted coarse-ground sweet white...
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A new type of soyabean, AC Proteus, containing higher protein than conventional cultivars was evaluated using Landrace x Hampshire pigs, weighing about 10 kg. In the first trial, AC Proteus was included at 0, 4, 8, 12, 16 or 20% of the isonitrogenous and isoenergetic soyabean meal-based diets and fe...
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Two feeding trials were conducted to evaluate lupin seed (Lupinus albus) as a replacement for soyabean meal at levels of 0, 5, 10, 15, 20 and 25% of starter diets fed to 192 pigs, from 10 to 20 kg liveweight. ADF and NDF concentrations in the diets increased as the proportion of lupin in the diet in...
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The N degradability and N digestibility of raw, roasted or extruded oilseeds were studied using an in vitro enzyme method. The N degradability and N digestibility of canola, linseed and soybean were calculated based on the proportional difference in N remaining after incubation and the initial N con...
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Effects of feeding heat-treated canola (C), soybean (S) and flax (F) or mixtures on growth and slaughter characteristics, taste and fatty acid (FA) composition of beef tissue were investigated using 128 crossbred steers to determine the potential of improving the nutritional quality of beef for huma...
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Lower fertility of preserved stallion semen may be caused by damage to the spermatozoa or premature capacitation during storage. We investigated the use of the flavonoid, quercetin, with a standard skim milk extender for storage of stallion spermatozoa to prevent premature capacitation and acrosome ...
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The effects of dietary lipid from 4 experimental diets on the fatty acid composition and cholesterol content of spermatozoa and their plasma membranes and their consequences for sperm viability after cryopreservation were evaluated in rainbow trout. The 4 sources of lipid were herring oil (adequate ...
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Seminal plasma protein of Arctic char Salvelinus alpinus was. characterized using sodium dodecyl sulfate (SDS) gel electrophoresis. Twelve protein bands with molecular weights of 7.2, 12.4, 15.3, 20.0, 20.4, 22.6, 39.4, 66.3, 74.0, 92.0, 94.5, and 130.1 kilodaltons (kDa) were detected. The effect of...
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The total fatty acid composition of rainbow trout (Oncorhynchus mykiss) spermatozoa and seminal plasma was investigated in fresh and in stored semen using gas liquid chromatography. Triglycerides, phospholipids, and cholesterol were measured and the effect of fatty acids, triglycerides, and choleste...
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The objective of this study was to maintain the viability of chilled rainbow trout (Oncorhynchus mykiss) eyed eggs during storage using oxygenated perfluorochemical (PFC). Three trials were conducted using eggs at 161, 180 or 217 degree days (days from fertilization x incubation temperature in degre...
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Cryopreservation of Arctic charr semen was investigated using 3 diluents (0.3 M glucose, 0.3 M glucose with 0.011 M KCl and 0.137 M NaCl, 0.011 M KCl, 0.004 M Na2HPO4 with 7.5 g/litre L- alpha -lecithin adjusted to pH 7.5) 3 cryoprotectants [10% dimethyl sulphoxide (DMSO), 10% dimethylacetamide (DMA...
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Mussel aquaculture on Prince Edward Island (PEI), Canada, is an important but relatively new industry. Although seed manipulation using hatcheries for mussel culture occurs on the west coast of North America, seed supply on the east coast of Canada, including Prince Edward Island (PEI), is based sol...
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Several media for the storage of fresh semen from rainbow trout at 4 degrees C were evaluated. Semen was diluted with an equal volume of fluorocarbon, a 1:1 fluorocarbon:extender emulsion, a 2:1 fluorocarbon:extender emulsion, or with extender, or was stored undiluted. The semen was stored under moi...
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Fifteen extenders were produced by adding dimethyl sulfoxide (DMSO) at 8, 10 or 12% of diluent volume to 5 diluents. All extenders were cooled to 4 degrees C. Pooled Atlantic salmon (Salmo salar ) semen with greater than 90% progressive motility was kept at 4 degrees C and added to each extender so ...
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The objective was to determine the oxidative stability of Arctic char (Salvelinus alpinus) semen following dietary supplementation with lowbush blueberry (Vaccinium angustifolium) product, alpha-tocopherol, alpha-tocopherol+blueberry product, or alpha-tocopherol+astaxanthin. Sperm lipid peroxidation...
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Dimethyl-acetamide was evaluated as a cryoprotectant for rainbow trout (Oncorhynchus mykiss ) semen on the basis of motility, percentage of dead spermatozoa as determined by fluorometry, and fertility of frozen-thawed spermatozoa. Dimethyl-acetamide performed significantly better (P<0.05) than th...
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