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An automated colorimetric method for determining lipase activity in canine sera was evaluated for precision, linearity and correlation to existing assay methods. The colorimetric method was a commercial reagent that used a series of enzymatic reactions based on the hydrolysis of 1,2 diglyceride by p...
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Four hundred eighty mature marine mussels (Mytilus edulis) were collected in late April from a mussel lease in an estuary in Prince Edward Island, Canada. Squash preparations made of reproductive gland (mantle) tissue of each mussel were stained with Wright-Giemsa stain and were examined by one inve...
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BACKGROUND: Evaluation of serum magnesium (Mg) concentration is becoming important in human and veterinary critical care medicine. An ion-selective electrode can measure the physiologically active ionized fraction. OBJECTIVES: The purpose of this study was to validate an ion-specific electrode analy...
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A homogenous enzyme immunoassay (EIA) for measurement of serum thyroxine (T4) concentration was evaluated for use with canine and feline serum. The EIA method was linear from 0 to 150 nmol T4/L for human serum, 0 to 94 nmol T4/L for feline serum and 10 to 60 nmol T4/L for canine serum. Intra- and in...
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Hyperthyroidism can increase the renal excretion of magnesium and thus cause hypomagnesemia in various species. Anaerobically collected blood samples from 15 hyperthyroid and 40 normal, healthy cats were analyzed with an ion-selective electrode analyzer and a serum biochemical analyzer. There was no...
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Serum C-reactive protein (CRP) concentration was measured, using an automated immunoturbidimetric assay, in 44 clinically normal dogs and 67 dogs with band neutrophil count > or = 10(9) cells/L, and values were found to be significantly (P or = 10(9) band neutrophils/L resulted in a statistically...
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A colorimetric method (glucose oxidase/peroxidase) for indirect measurement of glycogen concentrations in tissue homogenates of marine mussels (Mytilus edulis) was evaluated. This method uses a conversion of glycogen to glucose by amyloglucosidase. Varying the buffer pH (4.5, 5.0; 5.5) and the amylo...