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101 - 125 of 151
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Experimental infection of rainbow trout juveniles with L. salmonae at a water temperature of 15 degrees C yielded detectable parasite DNA within the gills by the second week after exposure (AE) and detectable spore-wall antigen within developing xenomas by week 3 AE, as determined by in situ hybridi...
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The principal lesions due to the ciliate Anophryoides haemophila in experimentally infected lobsters are observed in the gills and connective tissue from the 9th week post-infection. These lesions were not related to the inoculum of parasites and were consistent in all inoculum studies (2000, 10 000...
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Experiments involving 200 rainbow trout showed that host defence mechanisms in sea lice-infested fish are impaired, predisposing fish to subsequent infections to a second pathogen, such as the microsporidian parasite Loma salmonae. Macrophage impairment in lice-infested fish occurred when sea lice b...
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Gills from Atlantic salmon with experimentally induced amoebic gill disease (Neoparamoeba spp.) were examined with transmission electron microscopy to assess pathology and host-cell responses. Amoebae were found either on the surface epithelium or with pseudopodia extending deeply into invaginations...
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Chloramine-T is a commonly used therapeutic agent for the treatment of bacterial gill disease and related phenomena in the salmonid aquaculture industry. There is little known about its effects on healthy fish. Using a 24-tank replicate growth assay system, rainbow trout (Oncorhynchus mykiss) (avera...
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Whitish, opaque, coalescing masses were observed coating the visceral organs of numerous adult coho salmon when they were dissected for artificial spawning in the autumn of 1983 at the Puntledge River hatchery, British Columbia. One affected fish was observed in November 1991. Histological examinati...
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Juvenile brook trout (Salvelinus fontinalis), rainbow trout (Oncorhynchus mykiss), Atlantic salmon (Salmo salar) and Arctic charr (Salvelinus alpinus) were experimentally infected with a Loma salmonae variant to assess its infectivity within a range of salmonid species. The study shows that after in...
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The purpose of this research was to investigate the salinity and formalin sensitivity of a ciliate parasite (Anophryoides haemophila) of the American lobster (Homarus americanus), and to examine the target-animal (lobster) safety of chemical-bath treatments involving low salinity, formalin, or chlor...
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Healthy gills from fingerling rainbow trout were dissected and fixed using either a conventional 0.1M cacodylate buffered 2.5% glutaraldehyde or the same fixative with 2% (w/v) Alcian blue added. The tissues were processed for light and electron microscopy. Skin tissue samples from cases of necrotic...
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This study was designed to investigate the effect of single short-duration bath-treatment with hydrogen peroxide (1000, 1250 or 1500 mg/l for 20 min), as currently used in treatment for sea lice, on the growth rate and gill morphology of rainbow trout. All three dose levels significantly reduced the...
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Chronic mortalities in lumpfish Cyclopterus lumpus reared in a saltwater recirculation system were associated with the presence of an intranuclear microsporidian. Morphological changes were characterized by infiltration of lymphocyte-like cells predominantly in the renal interstitium and also in the...
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This study compared and contrasted the fate of the microsporidian Loma salmonae, a branchial pathogen of salmonids of the genus Oncorhynchus, upon exposure of (1) naive susceptible rainbow trout (RT) O. mykiss, (2) naive RT passively immunized with sera from RT previously exposed to L. salmonae, (3)...
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Loma salmonae, an obligate intracellular microsporidian parasite, is the causal agent of microsporidial gill disease of salmon (MGDS), characterized by the production, growth and eventual rupture of spore-filled xenomas. MGDS in farmed chinook salmon remains occult until xenoma rupture, at which tim...
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Three exposure studies were conducted to assess whether oral exposure of naive rainbow trout to Loma salmonae spores at 2 temperatures (10 and 14.5 degrees C) provides protection against disease upon rechallenge with spores; to repeat the study at 14.5 degrees C using fish which had recovered from e...
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Two trials were designed to quantify the effect of feeding ratio and fish size on the cohabitation transmission of Loma salmonae, the causative agent of microsporidial gill disease (MGD) in salmonids, Oncorhynchus spp. To evaluate the effect of feeding ratio on disease onset, groups of 45 rainbow tr...
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The main objective of this investigation was to examine the ultrastructural features of gills from rainbow trout experimentally infected with Loma salmonae to determine the morphological events that occur during the late stages of development of this parasite. Peripheral distribution of the mature p...
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The purpose of this study was to determine adequate dose ranges and to test for side effects associated with chronic treatment of fish with indomethacin. Rainbow trout Oncorhynchus mykiss were orally treated with indomethacin at various nominal concentrations: a negative control (0 mg/kg) and...
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The pathology caused by the ciliate Anophryoides haemophila was studied in experimentally infected lobsters. The first lesions due to the parasite were observed in gills and connective tissue from 9th week post-infection for the lower inocula and at week 4 for the 500,000 ciliate inoculum.. These le...
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Following a per os challenge of naive rainbow trout with live spores of Loma salmonae, head kidney mononuclear cells (MNC) in culture were able to proliferate in response to crude soluble parasite extract or intact dead spores. A significant response was seen by week 2 post-exposure and a maximum re...
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Plasmacytoid leukaemia (PL) is a lymphoproliferative disorder that affects netpen-reared chinook salmon in British Columbia, Canada. The only diagnostic test for PL is histological evaluation of suspect fish. Monoclonal antibodies (MAbs) previously reported to react with tissues of PL-affected fish ...
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Members of an Ahousaht First Nations community of Vancouver Island, Canada have developed a market for wild-caught species of indigenous rockfish (Sebastes spp.). The fish, which are caught by hook and line, are transported live to fish markets in Vancouver. Recently, market place downgrading of the...
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A new species of trichosomoidid nematode, Huffinanela canadensis n. sp., is described from the skin of rockfish (Sebastes spp.) from the Pacific Ocean off the coast of Vancouver Island (Clayoquot Sound region), British Columbia, Canada, on the basis of the morphology of the adult worms and their egg...
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Immune response to fish microsporidia is still unknown and there are current research trying to elucidate the events involved in the immune response to this parasite. There is evidence suggesting the role of innate immune response and it is clear that adaptive immunity plays an essential part for el...